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Freeze fracture

Freeze fracture

method of specimen preparation for the electron microscope in which rapidly frozen tissue is cracked so as to produce a fracture plane through the specimen. The surface of the fracture plane is then shadowed by heavy Metal vapour, strengthened by a carbon film and the underlying specimen is digested away, leaving a replica that can be picked up on a grid and examined in the transmission electron microscope. The great advantage of the method is that the fracture plane tends to pass along the centre of lipid bilayers and it is therefore possible to get en face views of membranes that reveal the pattern of integral membrane proteins. The e face is the outer lamella of the plasma membrane viewed as if from within the cell, the p face the inner lamella viewed from outside the cell. Fracture planes also often pass along lines of weakness such as the interface between cytoplasm and membrane, so that outer and inner membrane surfaces can be viewed. Further information about the structure can be revealed by freeze etching. Extremely rapid freezing followed by deep etching has allowed the structure of the cytoplasm to be studied without the artefacts that might be introduced by fixation.


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Tools for studying cells question

from wikipedia "Freeze-fracture or freeze-etch – a preparation method particularly useful for examining lipid membranes and their ...

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by MrMistery
Fri Dec 26, 2008 6:07 pm
 
Forum: Cell Biology
Topic: Tools for studying cells question
Replies: 2
Views: 4206

Tools for studying cells question

... This is part of a study effort for a big test coming up soon. What is freeze fracture? Freeze-etching? Could someone explain this to me? Progress I made: I have a book ...

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by thewax
Fri Dec 26, 2008 12:00 am
 
Forum: Cell Biology
Topic: Tools for studying cells question
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Views: 4206

The Fiber Disease

... upon their morphology adopted in a biological relevant environment. Freeze-fracture electron microscopy is not only a powerful technique to characterize drug/gene carrier ...

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by Nadas Moksha
Fri Dec 22, 2006 6:54 am
 
Forum: Human Biology
Topic: The Fiber Disease
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Re: Cell membrane

... is a mosaic of proteins inserted in the phospholipid bilayer(freeze fracture method prove it) Search Google or PubMed...

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by zami'87.
Sat Feb 11, 2006 10:29 pm
 
Forum: Cell Biology
Topic: Cell membrane
Replies: 3
Views: 1877

The Fiber Disease

... for sister Rose....: *Rosettes* Caulonema cell at the tip of Funaria: freeze fracture of the plasma membrane. The protein molecules are partially arranged in rosettes. ...

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by London
Fri Dec 02, 2005 11:43 am
 
Forum: Human Biology
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