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Freeze


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Mechanism behind Cyanobacterial Cryptobiosis?

... behind this feet are that they form something called microbial mats which include coatings that protect against UV, some form of organic 'anti-freeze', and that they begin recycling nutrients while lowering their metabolic rate. Which is all well and good, but it's not very specific. By comparison ...

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by NMLevesque
Fri Nov 22, 2013 5:54 pm
 
Forum: Microbiology
Topic: Mechanism behind Cyanobacterial Cryptobiosis?
Replies: 0
Views: 1292

Dioxin and Heated Bottled Water

... This information is also being circulated at Walter Reed Army Medical Center … No plastic containers in microwaves. No plastic water bottles in freezers. No plastic wrap in microwaves. Dioxin chemical causes cancer, especially breast cancer. Dioxins are highly poisonous to cells in our bodies. ...

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by Ahsmeah
Fri Aug 02, 2013 3:21 pm
 
Forum: General Discussion
Topic: Dioxin and Heated Bottled Water
Replies: 1
Views: 1432

Plasimd prep from frozen cells

Just grow the cells, spin them down, freeze and keep at -80°C until used.

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by JackBean
Fri Mar 29, 2013 2:07 pm
 
Forum: General Discussion
Topic: Plasimd prep from frozen cells
Replies: 1
Views: 1005

Problems making chemically competent cells

... Pre-chill a flask on ice for the chilling step • Pre-chill the centrifuge and its rotor • Work in the cold room! • Pre-chill eppendorfs in the -70 freezer, and tips in the cold room, (and maybe an eppendorf rack in the -80) 1. Make a 5ml pre-culture overnight from a single colony, in SOC medium ...

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by lilruthc
Fri Feb 08, 2013 1:09 pm
 
Forum: Molecular Biology
Topic: Problems making chemically competent cells
Replies: 1
Views: 2659

Commercial AFP and Osmotic Pressure Salt Water Fish

I am trying to improve our frozen fish business by adding AFP (Anti Freeze Protein). I have got a few questions I would like to ask: 1. Is Type III AFP commercially available? 2. If so, where / who should I contact? 3. Otherwise, any better or more readily ...

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by anthell
Tue Nov 20, 2012 6:37 pm
 
Forum: Molecular Biology
Topic: Commercial AFP and Osmotic Pressure Salt Water Fish
Replies: 0
Views: 1818
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