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Free electrophoresis

Free electrophoresis

electrophoresis of substances placed in a solution in a U-shaped tube.

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PCR from cDNA question

... marine species using QIAGEN RNeasy Plus mini kit eluted with 40ul RNase free water. Nanodrop gives a low reading and a weak absorption peak (~10ng/ul). ... ribosomal RNAs). The RNA sample have been heated at 65C for 5min before electrophoresis to eliminate hairpin structures. The cDNA was obtained ...

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by vastgenome
Fri Jun 22, 2012 3:55 pm
Forum: Molecular Biology
Topic: PCR from cDNA question
Replies: 1
Views: 4085

Re: SDS-PAGE help please

... seems that there is a little confusion about what we are taking about. Electrophoresis is a process used for the separation of cellular proteins ... the polymerization of the acrylamide polymers by enhancing the free radical production of APS, which results in the gel matrix. The size ...

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by MJD
Thu Oct 01, 2009 2:58 am
Forum: Bioinformatics
Topic: SDS-PAGE help please
Replies: 6
Views: 14477

Re: Matching 2D membrane spots to the gel

Hello, I work on similar experiments. Our group works on 2D-electrophoresis of wheat and zea embryos. For this experiments I use methods ... a place of presence my protein according to pI and Mr my protein. Feel free to ask for more Jana

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by JackBean
Mon Sep 21, 2009 8:39 pm
Forum: Molecular Biology
Topic: Matching 2D membrane spots to the gel
Replies: 1
Views: 1852

How to improve DNase efficiency?

... to get rid of all DNA. After that, we purified our RNAs. By PCR and electrophoresis, we found out that we still had DNA with our RNA. Therefore ... PCR) that not all the DNA was digested. We first used the RNase free DNase 1 (2U/µl), mixing 1µl with 19µl of the products of the in vitro ...

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by soam
Wed Jul 23, 2008 12:15 pm
Forum: Molecular Biology
Topic: How to improve DNase efficiency?
Replies: 0
Views: 987

The Fiber Disease

... transfection protocol Separation of proteins by polyacrylamide gel Electrophoresis Preparation of protein probes EXPERIMENTAL What, then, determines ... the relationship between surface area and hydrolytic rate in cell-free systems is rather equivocal. By contrast, the relationship between available ...

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by Nadas Moksha
Wed Dec 20, 2006 9:34 pm
Forum: Human Biology
Topic: The Fiber Disease
Replies: 7403
Views: 6245092
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