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Fragments

Fragment

a small part broken from a larger entity.


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Souther blot and restriction mapping

... fragment data obtained through endonuclease digestion and separation by standard gel electrophoresis, however, Southern Blot data only shows those fragments that a probe has hybridised to and I am struggling to find the correct approach. The parting words from lecturer were 'It's just a matter ...

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by Mistersnuffle
Sat Oct 04, 2014 11:17 am
 
Forum: Genetics
Topic: Souther blot and restriction mapping
Replies: 0
Views: 21

Re: MEG-01 cell line cultivation Problem!!!

I think I have the same problem with MEG 01. I defrost them 3 days ago and they are with many fragments suspended in media with some attached cells. I did not notice an obvious in growth until today. This is the first time I work with MEG 01. How could I differentiate ...

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by Enaam
Fri Oct 03, 2014 9:11 am
 
Forum: Cell Biology
Topic: MEG-01 cell line cultivation Problem!!!
Replies: 1
Views: 1325

Re: Gel electrophoresis

haha, so there is a high concentration of the small fragments?

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by downingrock
Thu Mar 13, 2014 1:08 am
 
Forum: Genetics
Topic: Gel electrophoresis
Replies: 3
Views: 1764

Okazaki Fragment and RNA Primer

Hi, I have a simple semantics question. I have been reading some literature that seems to consider RNA Primers as part of Okazaki fragments, but this should not be so because Okazaki Fragments by definition are just the DNA portions synthesized on the lagging strand. Any opinions/clarifications ...

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by ahyaa
Sun Nov 17, 2013 10:01 am
 
Forum: Molecular Biology
Topic: Okazaki Fragment and RNA Primer
Replies: 1
Views: 1402

Promoter PCR primer set problem

... Both your enzymes are fine with only 1 base in the end: https://www.neb.com/tools-and-resources/usage-guidelines/cleavage-close-to-the-end-of-dna-fragments

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by JackBean
Tue Jul 09, 2013 9:29 am
 
Forum: Molecular Biology
Topic: Promoter PCR primer set problem
Replies: 2
Views: 2341
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