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Fragments

Fragment

a small part broken from a larger entity.


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Re: Gel electrophoresis

haha, so there is a high concentration of the small fragments?

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by downingrock
Thu Mar 13, 2014 1:08 am
 
Forum: Genetics
Topic: Gel electrophoresis
Replies: 3
Views: 1591

Okazaki Fragment and RNA Primer

Hi, I have a simple semantics question. I have been reading some literature that seems to consider RNA Primers as part of Okazaki fragments, but this should not be so because Okazaki Fragments by definition are just the DNA portions synthesized on the lagging strand. Any opinions/clarifications ...

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by ahyaa
Sun Nov 17, 2013 10:01 am
 
Forum: Molecular Biology
Topic: Okazaki Fragment and RNA Primer
Replies: 1
Views: 1300

Promoter PCR primer set problem

... Both your enzymes are fine with only 1 base in the end: https://www.neb.com/tools-and-resources/usage-guidelines/cleavage-close-to-the-end-of-dna-fragments

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by JackBean
Tue Jul 09, 2013 9:29 am
 
Forum: Molecular Biology
Topic: Promoter PCR primer set problem
Replies: 2
Views: 2279

Asking for help with electrophoresis

... agarose was used - resolving power of that is usually 200bp and up. Increasing the percentage of agarose would improve resolution of smaller fragments somewhat, but if GOOD resolution is required, genetic grade agarose is needed.

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by Cat
Sun Jun 09, 2013 4:47 pm
 
Forum: Molecular Biology
Topic: Asking for help with electrophoresis
Replies: 5
Views: 2223

Asking for help with electrophoresis

1. It seems that you are using regular agarose which separates 200bp and up. You need genetic analysis grade agarose that allows good separation of fragments bellow 1kb. And even with that, you will need to make a 2-3% agarose gel. Here is the link to the agarose that you need: http://www.fishersci.com/ecomm/servlet/fsproductdetail?storeId=10652&productId=14260139&catalogId=29104&matchedCatNo=BP1360100&fromSearch=1&searchKey=agarose&highlightProductsItemsFlag=Y&endecaSearchQuery=%23store%3DScientific%23nav%3D4294876167%23rpp%3D25%23keyWord%3Dagarose&xrefPartType=From&savings=0.0&xrefEvent=1370706282459_7&searchType=PROD ...

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by Cat
Sat Jun 08, 2013 3:57 pm
 
Forum: Molecular Biology
Topic: Asking for help with electrophoresis
Replies: 5
Views: 2223
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