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Fragment reaction

Fragment reaction

a reaction used to assay the activity of peptidyl transferase.


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Primer dimer

... (i) PCR Clean-up: A clean-up method to remove dNTPs, short oligo fragments, mineral oil, enzymes from a PCR reaction product. It does not eliminate unspecific bands and primer-dimers. The quality of ...

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by Nmr
Tue Nov 15, 2011 3:42 pm
 
Forum: Molecular Biology
Topic: Primer dimer
Replies: 1
Views: 1320

Not understanding "rendered flush" concerning vectors

... this often not the case, it is necessary to blunt the ends- if Klenow fragment of DNA polymerase is used (E.coli DNA polymerase I lacking the proofreading ... ends into the blunt ended vector. This is a more difficult cloning reaction than a cohesive-end cloning reaction.

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by merv
Sun Oct 02, 2011 3:03 pm
 
Forum: Molecular Biology
Topic: Not understanding "rendered flush" concerning vectors
Replies: 4
Views: 4320

Re: genomic rescue construct from BAC DNA

If I were you, I wouldn't just add more DNA to the reaction. I've come to believe that if you can see it, you can clone it. Are you ... are the enzymes from? One thing you can do is treat one of your linear fragments with Calf Intestinal Alkalyn Phosphatase (CIP). This will stop re-ligation ...

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by antonyviridae
Wed Sep 07, 2011 8:33 pm
 
Forum: Molecular Biology
Topic: genomic rescue construct from BAC DNA
Replies: 2
Views: 3521

Re: How to isolate specific dna fragment?

And can i use primers for pcr without electrophoresis ? You must use primers for PCR, no matter, what you do with the reaction afterwards ;) You can e.g. purify the amplicon by some spin columns or directly digest with RE and afterwards purify etc. ;)

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by JackBean
Fri Aug 26, 2011 12:18 pm
 
Forum: Genetics
Topic: How to isolate specific dna fragment?
Replies: 7
Views: 4531

This might be a stupid question!! But I will ask anyways :D

... of PCR is in this movie. Generally PCR amplify one or only a few fragment (multiplex PCR) in one reaction, although some can go way beyond either because of the awesome technical skill of ...

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by canalon
Thu Jun 09, 2011 10:47 pm
 
Forum: Genetics
Topic: This might be a stupid question!! But I will ask anyways :D
Replies: 4
Views: 2833
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