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Fragment

Fragment

a small part broken from a larger entity.


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Basic Knowledge about DNA Extraction

... Fluorescence In Situ Hybridization (FISH), a molecular technique used to identify and enumerate specific bacterial groups and Terminal Restriction Fragment Length Polymorphism (T-RFLP), the technique used to identify, characterize and quantify spatial and temporal patterns in marine bacterioplankton ...

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by 2444256703
Thu Jan 22, 2015 8:56 am
 
Forum: Genetics
Topic: Basic Knowledge about DNA Extraction
Replies: 0
Views: 749

Souther blot and restriction mapping

... B + E gives |-----------2,0---------|------------1,6-------|----0,2------| if you compare XE and XB, you can get either 1.6 fragment in between (with my positioning) or 0.6 fragment with the opposite position of fragments in XE (i.e. |--1,7--|--0,7--| ). Now you just have ...

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by JackBean
Wed Oct 08, 2014 9:57 am
 
Forum: Genetics
Topic: Souther blot and restriction mapping
Replies: 1
Views: 1585

Souther blot and restriction mapping

... I would greatly appreciate any help with constructing a restriction map from Southern Hybridisation data. I can construct restriction maps from fragment data obtained through endonuclease digestion and separation by standard gel electrophoresis, however, Southern Blot data only shows those fragments ...

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by Mistersnuffle
Sat Oct 04, 2014 11:17 am
 
Forum: Genetics
Topic: Souther blot and restriction mapping
Replies: 1
Views: 1585

Re: microsatellite instability

OK, another question: I can see how insertion/deletion can be detected by fragment analysis, but incorporation of a wrong nucleotide wouldn't cause change in the length of amplicon, so can that also be detected by fragment analysis?

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by kk
Thu Jul 10, 2014 2:45 pm
 
Forum: Molecular Biology
Topic: microsatellite instability
Replies: 3
Views: 3447

No "beginning-replication problem" on leading strand?

... those a continuous strand is produced until meeting replicating fork from another direction or end of the strand. The primer is removed by Okazaki fragment going in the other direction.

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by JackBean
Tue May 13, 2014 4:55 pm
 
Forum: Molecular Biology
Topic: No "beginning-replication problem" on leading strand?
Replies: 1
Views: 1905
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