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Fragment

Fragment

a small part broken from a larger entity.


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Re: microsatellite instability

OK, another question: I can see how insertion/deletion can be detected by fragment analysis, but incorporation of a wrong nucleotide wouldn't cause change in the length of amplicon, so can that also be detected by fragment analysis?

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by kk
Thu Jul 10, 2014 2:45 pm
 
Forum: Molecular Biology
Topic: microsatellite instability
Replies: 3
Views: 2342

No "beginning-replication problem" on leading strand?

... those a continuous strand is produced until meeting replicating fork from another direction or end of the strand. The primer is removed by Okazaki fragment going in the other direction.

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by JackBean
Tue May 13, 2014 4:55 pm
 
Forum: Molecular Biology
Topic: No "beginning-replication problem" on leading strand?
Replies: 1
Views: 713

No "beginning-replication problem" on leading strand?

... is always so that we can replicate the end of the lagging strand. This is because the extreme end have a RNA primer to make the last Okazaki fragment, and after the removal of this primer it needs to somehow be replaced with DNA. I have the latest version of Molecular Biology of the Gene ...

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by poobear
Fri Apr 11, 2014 9:40 pm
 
Forum: Molecular Biology
Topic: No "beginning-replication problem" on leading strand?
Replies: 1
Views: 713

Gel electrophoresis

Hey guys! Would anyone know, in each of the Lane 2's, why there is a very large bright fragment that doesn't really confine to the shape of a rectangle? Just a general description would be helpful. Thank you!

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by downingrock
Wed Mar 12, 2014 12:44 pm
 
Forum: Genetics
Topic: Gel electrophoresis
Replies: 3
Views: 1591

Okazaki Fragment and RNA Primer

Hi, I have a simple semantics question. I have been reading some literature that seems to consider RNA Primers as part of Okazaki fragments, but this should not be so because Okazaki Fragments by definition are just the DNA portions synthesized on the lagging strand. Any opinions/clarifications on t...

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by ahyaa
Sun Nov 17, 2013 10:01 am
 
Forum: Molecular Biology
Topic: Okazaki Fragment and RNA Primer
Replies: 1
Views: 1301
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