Fractions

... x Z x 1/4] - {[Z x Z x 1/4] x [Z x Z x 1/4]} The part i don't really get is the Probability to get a heterozygote P(Ff). Why is multiplied by two fractions instead of one? ie: why is it (1/2 x 1/2) instead of just 1/2 for say the cross FFxFf.

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You're going to get 4 fractions when you're doing this Cytosolic proteins Membrane proteins Nuclear Proteins Cytoskeletal proteins For example in the Qproteome kit it breaks open the plasma membrane but doesn't solubilise it you spin ...

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... properly explained. We did a VERY QUICK example in class that was similar but all we did was take the mother and father genotypes and come up with fractions for possibilities. There weren't any unknowns for the parents and when I went back and looked at the diagram, it didn't even make sense. My ...

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do you have some controls on your Western (to make sure, your fractions are pure)?

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Hope I'm making sense! not really but if your question is simply, whether you can pool fractions with your protein, than of course yes. Just take care so that you won't pool all your franctions, then would be the chromatography quite useless. It depends, what is your ...

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