Fractionation

... bonds? - what is the relationship between dehydration rxns and hydrolysis? - where would you find the alpha helix in a protein? - what is cell fractionation used for? - 2 atoms that are equal in electronegativity form what? - know which acids are pyrimidines and which are purines **hint: remember ...

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Electron microscopy? That's kind of a pain and I don't know what kind of shape they'll be in after isolation. You could do enzyme assays to confirm (Krebs cycle enzymes, maybe) and, to some extent rule out contamination (some glycolytic enzyme or DNase maybe? not sure what people usually use.).

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how could the contents of a pellet be examined to confirm that it contains mainly mitochondria?? thankksss

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... faster to pellet the next-most-dense fraction, and so on to the least dense. There is a kit (at least one—there may be others) for routine cell fractionation. The kits are commonly used to prepare microsomes to test potential drug candidates for the ability to induce Cyto-P450 in liver cells. ...

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... plus some membrane fraction contamination my hint - DO use protease inhibitors throughout the procedure my own small scale lab protocol for staph fractionation is as follows: 1. grow the strain in an appropriate medium (TSB or BHI) at 37C/200 rpm till stationary phase (overnight) and collect the ...

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