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Fraction i protein

Fraction i protein

(Science: protein) see ribulose bisphosphate carboxylase/oxidase RUBISCO).

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Re: Protein expression but no mRNA

Sorry for not responding sooner. The protein is indeed present in serum. A strong band is detected both in patients and ... Maybe, the protein is simply bound to cell surface receptors?!? But when I do fractionation studies, the protein is detected in the cytosollic fraction. More ideas?

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by evamik
Thu Jun 07, 2012 8:02 am
Forum: Molecular Biology
Topic: Protein expression but no mRNA
Replies: 10
Views: 7136

Extracting proteins from foliage

Jack, Won't membranes also trap any undesirable large species with the protein fraction? Thought it was better to precipitate proteins out selectively.

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by Jeanmaxwell
Fri May 18, 2012 12:10 am
Forum: Botany Discussion
Topic: Extracting proteins from foliage
Replies: 4
Views: 2315

Wrong imidazole conc

Well from what I understand, 66mM of imidazole was enough to elute your protein so you do not need to add more, and you can use your elution to do ... presume) but were not checking for the presence of the protein in each fraction to determine at which concentration the protein was leaving your ...

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by canalon
Mon Oct 31, 2011 10:04 am
Forum: Molecular Biology
Topic: Wrong imidazole conc
Replies: 3
Views: 1662


... into mature collagen fibrils. We further hypothesized that secreted protein acidic and rich in cysteine (SPARC) plays a key role in the changes ... LV collagen content was measured histologically by collagen volume fraction, collagen composition was measured by hydroxyproline assay as soluble ...

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by HerrBiologie
Sun Oct 30, 2011 9:12 am
Forum: Molecular Biology
Topic: hydroxyproline
Replies: 2
Views: 2037


... logic. :D Until we know the coding sequence its hard to assume the protein length. As we can easily calculate the number of amino acids i think ... of it - ) using the new exon in combination with the old promoter some fraction of its 'usual' exons - yet if it is a different function why call ...

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by merv
Mon Oct 03, 2011 12:06 am
Forum: Genetics
Topic: can protein size be predicted given genome info?
Replies: 8
Views: 9054
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