Fplc

If you're using an fplc instrument, you're better off using a gradient of imidazole for elution. Then your stuff will elute at whatever minimal concentration of imidazole that suits it. You can also try using some other chelation matrix. ...

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... obtained by standard protein purification techniques, including precipitation, column chromatography and fast performance liguid chromatography ()FPLC, and ultrafiltration A liquots of this fraction in buffer were added to the cloned cells in vitro in this buffered solution under controlled conditions. ...

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