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Fplc

Fplc

(Science: abbreviation) fast protein liquid chromatography.


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Buffer Considerations when using Anion Exchange (FPLC)

Hi Everyone! Quick question about protein purification using Anion Exchange column, I'm a little new to the equiptmnt and protein purification. I have a HIS-tagged protein that has a pI above 7.5. I'm using a 1M Tris, 50mM EDTA buffer at pH 7.5. So that means in this buffer my protein will have a ne...

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by dinofernando
Tue Feb 23, 2010 12:01 pm
 
Forum: Molecular Biology
Topic: Buffer Considerations when using Anion Exchange (FPLC)
Replies: 2
Views: 1595

Ni-NTA-Columns

If you're using an fplc instrument, you're better off using a gradient of imidazole for elution. Then your stuff will elute at whatever minimal concentration of imidazole that suits it. You can also try using some other chelation matrix. ...

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by blcr11
Thu Feb 14, 2008 12:09 pm
 
Forum: Molecular Biology
Topic: Ni-NTA-Columns
Replies: 3
Views: 2284

It is regarding mammalian cell growth in vitro, help me out

... obtained by standard protein purification techniques, including precipitation, column chromatography and fast performance liguid chromatography ()FPLC, and ultrafiltration A liquots of this fraction in buffer were added to the cloned cells in vitro in this buffered solution under controlled conditions. ...

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by heywhatever
Thu Jul 20, 2006 4:01 am
 
Forum: Cell Biology
Topic: It is regarding mammalian cell growth in vitro, help me out
Replies: 2
Views: 1241


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