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Formazan

Formazan

(Science: chemical) a water-insoluble coloured azo compound of the general structure, RNH-N==CR'-N==NR', formed by reduction of a tetrazolium salt in the histochemical demonstration of oxidative enzymes

Employing this reaction, oxidoreductase activity can be determined quantitatively in tissue sections by allowing the enzymes to act on their specific substrates in the presence of tetrazolium salts.

The R's are usually phenyl groups; examples include neotetrazolium, blue tetrazolium, and nitro blue tetrazolium.

Pharmacological action: indicators and reagents.


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The Fiber Disease

... bromide) identifies metabolic activity through its reduction by cellular dehydrogenases to microscopically visible deposits of insoluble formazan. MTT-negativity has earlier been shown to correlate with absence of growth of yeast and mycelial fungi in culture and could thus indicate the ...

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by tamtam
Mon Nov 27, 2006 3:26 pm
 
Forum: Human Biology
Topic: The Fiber Disease
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The Fiber Disease

... bromide) identifies metabolic activity through its reduction by cellular dehydrogenases to microscopically visible deposits of insoluble formazan. MTT-negativity has earlier been shown to correlate with absence of growth of yeast and mycelial fungi in culture and could thus indicate the ...

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by Nadas Moksha
Mon Nov 27, 2006 4:41 am
 
Forum: Human Biology
Topic: The Fiber Disease
Replies: 7403
Views: 5565905


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