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Fluorescent dyes

Fluorescent dyes

dyes that emit light when exposed to light. The wave length of the emitted light is usually longer than that of the incident light. Fluorochromes are substances that cause fluorescence in other substances, i.e., dyes used to mark or label other compounds with fluorescent tags. They are used as markers in biochemistry and immunology.


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hybridization probe

... with hybridization probes. I designed the probes but I couldn't choose fluorescent dyes, and don't know the criterias. Could you please help me...

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by FDHeLiX
Mon Sep 16, 2013 3:33 pm
 
Forum: Genetics
Topic: hybridization probe
Replies: 2
Views: 1118

looking for dyes that won't stain cells

Hi all, I'm looking for a recommendation: I need a cheap fluorescent dye that won't stain any part of my NIH-3T3 cells(a little endocytosis ... do tend to get through the membrane of unhealthy cells. Some of the alexa dyes or Fluorescein-dextran on the other hand would work but they cost too much ...

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by tigerrat
Tue Jan 10, 2012 2:14 am
 
Forum: Cell Biology
Topic: looking for dyes that won't stain cells
Replies: 0
Views: 737

Emission Intensity Profiles

(1)(a) A proton gradient can be analyzed using fluorescent dyes whose emission intensity profiles depend upon pH. One such dye, useful for measuring the pH gradient ...

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by niravana21
Wed May 04, 2011 8:51 pm
 
Forum: Cell Biology
Topic: Emission Intensity Profiles
Replies: 1
Views: 2138

Suspension cell

FACS and live/dead cell fluorescent dyes (like propidium iodide) It is quick and easy if you have acess to this kind of machine. Otherwise no ...

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by canalon
Fri Nov 12, 2010 5:40 pm
 
Forum: Cell Biology
Topic: Suspension cell
Replies: 1
Views: 1499

Ethidium Bromide (EtBr) or safe dyes???

Most people use Ethidium Bromide (EtBr) as a fluorescent dye for DNA. People know it is poisonous and dangerous to cause cancer. ... excite EtBr, which is risky for eyes and skins. Some people start to use safe dyes, like SyBr Safe, Gelgreen, Gelred, etc. They are safer but more expensive than ...

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by swing0916
Mon Oct 18, 2010 11:48 pm
 
Forum: Molecular Biology
Topic: Ethidium Bromide (EtBr) or safe dyes???
Replies: 3
Views: 1840
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