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Fluorescent

Fluorescent

Having the ability to emit light of a certain wavelength when activated by light of another wavelength.


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Re: Please help me decide which microscope to buy

... you need high-resolution images, down to the level of organelle structure, you will likely need to stain the cells anyway and will probably need a fluorescent microscope. A working cell bio lab is likely to have both inverted and traditional microscopes, likely with fluorescent capability in both ...

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by jonmoulton
Wed Nov 06, 2013 8:00 pm
 
Forum: General Discussion
Topic: Please help me decide which microscope to buy
Replies: 2
Views: 790

Measuring ROS with DCFH probe

... posting on this forum and I would really appreciate your opinions on the folllowing situation. I'm trying to measure ROS in HUVEC cells with the fluorescent probe DCFH by flow cytometry. I apply oxidant and antioxidant treatments, and what I get is that the cells with an oxidant treatment show ...

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by rem
Wed Oct 30, 2013 6:49 pm
 
Forum: Molecular Biology
Topic: Measuring ROS with DCFH probe
Replies: 1
Views: 501

hybridization probe

I will use lightcycler 2.0. to detect and SNP with hybridization probes. I designed the probes but I couldn't choose fluorescent dyes, and don't know the criterias. Could you please help me...

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by FDHeLiX
Mon Sep 16, 2013 3:33 pm
 
Forum: Genetics
Topic: hybridization probe
Replies: 2
Views: 1079

Gene deletion

Use the same protocol, just modify it - substitute Luciferase or other fluorescent protein gene in place of an antibiotic resistance gene.

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by Cat
Sun Sep 15, 2013 3:47 pm
 
Forum: Molecular Biology
Topic: Gene deletion
Replies: 5
Views: 1452

Overexpression

... about the vector providers (I'm in France)? - When I will make the transfection, I will need to select the transfected cells : is it better to use fluorescent proteins (as GFP) or antibiotics ? - A lot of protocol are designed for cell line macrophage but not for primary cells as BMDM. Is everyone ...

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by aureliegineste
Wed Sep 04, 2013 7:48 am
 
Forum: Molecular Biology
Topic: Overexpression
Replies: 1
Views: 675
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