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Fluorescent

Fluorescent

Having the ability to emit light of a certain wavelength when activated by light of another wavelength.


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gene knockout mice

... is not possible. You might combine genetics with viral infection. You might produce an AAV containing the Cre under an astrocytic promoter and a fluorescent marker and inject stereotactically in the striatum. This will not be 100% efficient, but might be sufficient for certain experiments. With ...

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by animodel123
Wed Mar 18, 2015 6:46 am
 
Forum: Genetics
Topic: gene knockout mice
Replies: 1
Views: 479

Basic Knowledge about DNA Extraction

... be found at the interface between the two phases. Finally, the presence of DNA can be confirmed by electrophoresing on an agarose gel or another fluorescent dye that reacts with the DNA, and also by UV light. Lab instruments used in DNA Extraction Bead beater is often used for DNA extraction, ...

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by 2444256703
Thu Jan 22, 2015 8:56 am
 
Forum: Genetics
Topic: Basic Knowledge about DNA Extraction
Replies: 0
Views: 794

I have got some BIG issues! forgive the long post

... ado: Here they are: Indirect DNA testing : I know yo target the genes that are in severe proximity to our targeted gene and that you make use a fluorescent nucleotide or a tagged reporter molecule that will attach themselves to those genes and fluorescent in order to show presence of the bad ...

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by saddy51
Sun Jan 04, 2015 10:39 am
 
Forum: Genetics
Topic: I have got some BIG issues! forgive the long post
Replies: 1
Views: 1961

Re: The most boring tasks in Biology

Counting cyanobacteria using a hemocytometer on a fluorescent microscope was boring. I was measuring the change in photosynthetic characteristics after changes in illumination at different iron concentrations. This involved lots of time points where ...

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by jonmoulton
Fri Aug 15, 2014 3:32 pm
 
Forum: General Discussion
Topic: The most boring tasks in Biology
Replies: 1
Views: 2284

Re: Please help me decide which microscope to buy

... you need high-resolution images, down to the level of organelle structure, you will likely need to stain the cells anyway and will probably need a fluorescent microscope. A working cell bio lab is likely to have both inverted and traditional microscopes, likely with fluorescent capability in both ...

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by jonmoulton
Wed Nov 06, 2013 8:00 pm
 
Forum: General Discussion
Topic: Please help me decide which microscope to buy
Replies: 2
Views: 2798
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