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Fluorescence microscopy

Definition

noun

A technique using a light microscope to study properties of organic or inorganic substances using the phenomena of fluorescence and phosphorescence instead of, or in addition to, reflection and absorption.


Supplement

Fluorescence microscopy is used in FISH analysis where a fluorescent probe is located if bound to specific DNA sequence that is complementary to it.


See also: FISH

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Photosynthesis

... chemicals by spectrophotometric assays. you can monitor organelles by fluorescence microscopy etc. What exactly are you talking about? Your questions are so general that it is ...

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by MrMistery
Wed Aug 12, 2009 11:23 pm
 
Forum: Cell Biology
Topic: Photosynthesis
Replies: 10
Views: 287

Re: Difference between ICC and IF

... processes made cells permeable). The fluorochrome is detected by fluorescence microscopy, always involving excitation of fluoresence with a defined wavelength band of light ...

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by jonmoulton
Tue Sep 16, 2008 3:37 pm
 
Forum: Molecular Biology
Topic: Difference between ICC and IF
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Views: 760

Re: UV Microscopy

... than the emitted or fluorescent light. If the excitation wavelength is to near the emitted wavelength there is not enough contrast to see the fluorescence.

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by Fauna
Mon Jul 28, 2008 9:24 pm
 
Forum: General Discussion
Topic: UV Microscopy
Replies: 7
Views: 915

The Fiber Disease

... from a novel clade of parasites that infect fish and amphibians. Fluorescence in situ hybridization and R. seeberi-specific PCR showed that ... stained with Periodic acid-Schiff (PAS) and visualized by bright-field microscopy at 400X magnification. The thick walls of immature R. seeberi ...

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by al
Mon Dec 25, 2006 1:39 am
 
Forum: Human Biology
Topic: The Fiber Disease
Replies: 7403
Views: 749540

The Fiber Disease

... as N^0.61, which is close to the strong segregation limit scaling. By fluorescence video imaging of worm dynamics, we also show that the persistence ... adopted in a biological relevant environment. Freeze-fracture electron microscopy is not only a powerful technique to characterize drug/gene carrier ...

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by Nadas Moksha
Fri Dec 22, 2006 6:54 am
 
Forum: Human Biology
Topic: The Fiber Disease
Replies: 7403
Views: 749540
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