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Fluorescence

Fluorescence

(Science: chemistry, physics) The emission of one or more photons by a molecule or atom activated by the absorption of a quantum of electro magnetic radiation.

Typically the emission, that is of longer wavelength than the excitatory radiation, occurs within 10exp 8 seconds: phosphorescence is a phenomenon with a longer or much longer delay in re radiation. Note that rays, x-rays, uv, visible light and ir radiations may all stimulate fluorescence.


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Measuring ROS with DCFH probe

... probe DCFH by flow cytometry. I apply oxidant and antioxidant treatments, and what I get is that the cells with an oxidant treatment show less fluorescence than the control and those with oxidant + antioxidant. The curious thing is that the fluorescence pattern I see is exactly opposite to ...

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by rem
Wed Oct 30, 2013 6:49 pm
 
Forum: Molecular Biology
Topic: Measuring ROS with DCFH probe
Replies: 1
Views: 1324

hybridization probe

... much. You can use this link to help you with that: http://www.lifetechnologies.com/us/en/home/life-science/cell-analysis/labeling-chemistry/fluorescence-spectraviewer.html You can try this pair - Fluorescein (FITC)/ROX (carboxy-X-rhodamine).

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by Cat
Sun Sep 22, 2013 3:37 pm
 
Forum: Genetics
Topic: hybridization probe
Replies: 2
Views: 1568

Stem Cells

... markers (cluster of differentiation; CD) that can also be used to track down these cells, either with flow cytometry or other monoclonal antibody/fluorescence-based assays. The problem is that the stem cells are usually very rare and thus difficult to detect, and in many cases the CD markers are ...

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by biohazard
Tue May 14, 2013 6:56 am
 
Forum: Molecular Biology
Topic: Stem Cells
Replies: 4
Views: 2574

Re: Device for ratiometric fluorescence measurement

If you don't mind losing the flexibility of scanning the spectrum or rapidly changing ex/em bands, you would build a 2-band fluorescence colorimeter. You'd need two emission filters and one or two excitation filters, arranged around your cuvette so that the excitation and emission light ...

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by jonmoulton
Mon Mar 04, 2013 4:58 pm
 
Forum: General Discussion
Topic: Device for ratiometric fluorescence measurement
Replies: 1
Views: 1433

Device for ratiometric fluorescence measurement

Hi, In the lab,I use fluorescence spectrometer for the ratiometric fluorescence measurement which needs to measure optical intensity at two different wavelenghts. However, the fluorescence spectrometer is expensive and huge. I am wondering ...

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by LittleMike
Mon Mar 04, 2013 3:40 pm
 
Forum: General Discussion
Topic: Device for ratiometric fluorescence measurement
Replies: 1
Views: 1433
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