Dictionary » F » Fluorescence



(Science: chemistry, physics) The emission of one or more photons by a molecule or atom activated by the absorption of a quantum of electro magnetic radiation.

Typically the emission, that is of longer wavelength than the excitatory radiation, occurs within 10exp 8 seconds: phosphorescence is a phenomenon with a longer or much longer delay in re radiation. Note that rays, x-rays, uv, visible light and ir radiations may all stimulate fluorescence.

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Taqman probe design using FISH probe (need help)

... PCR extension temperature (but not so high as a fish probe) and will be hydrolysed by Taq DNA polymerase at every replication, thus releasing the fluorescence (quenched in intact probe) that will be measured to quantify the starting level of template. Therefore, you can design primers and probes ...

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by protoemics
Wed Feb 04, 2015 8:11 am
Forum: Bioinformatics
Topic: Taqman probe design using FISH probe (need help)
Replies: 2
Views: 543

Basic Knowledge about DNA Extraction

... as well as to diagnose disease and genetic disorders. Many techniques in biological research are involved with the extraction of DNA, such as Fluorescence In Situ Hybridization (FISH), a molecular technique used to identify and enumerate specific bacterial groups and Terminal Restriction Fragment ...

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by 2444256703
Thu Jan 22, 2015 8:56 am
Forum: Genetics
Topic: Basic Knowledge about DNA Extraction
Replies: 0
Views: 750

Flow cytometry

Hi there, So I have carried out flow three times now to measure fluorescence (each time one sample per what I was looking at i.e no triplicates or anything)...and each of the three times the exp was carried out the same way. Would it be OK to combine the ...

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by biology_06er
Mon Oct 13, 2014 11:37 pm
Forum: Molecular Biology
Topic: Flow cytometry
Replies: 0
Views: 477

Measuring ROS with DCFH probe

... probe DCFH by flow cytometry. I apply oxidant and antioxidant treatments, and what I get is that the cells with an oxidant treatment show less fluorescence than the control and those with oxidant + antioxidant. The curious thing is that the fluorescence pattern I see is exactly opposite to ...

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by rem
Wed Oct 30, 2013 6:49 pm
Forum: Molecular Biology
Topic: Measuring ROS with DCFH probe
Replies: 1
Views: 1838

hybridization probe

... much. You can use this link to help you with that: http://www.lifetechnologies.com/us/en/home/life-science/cell-analysis/labeling-chemistry/fluorescence-spectraviewer.html You can try this pair - Fluorescein (FITC)/ROX (carboxy-X-rhodamine).

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by Cat
Sun Sep 22, 2013 3:37 pm
Forum: Genetics
Topic: hybridization probe
Replies: 2
Views: 2091
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