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Dictionary » F » Fluorescence

Fluorescence

Fluorescence

(Science: chemistry, physics) The emission of one or more photons by a molecule or atom activated by the absorption of a quantum of electro magnetic radiation.

Typically the emission, that is of longer wavelength than the excitatory radiation, occurs within 10exp 8 seconds: phosphorescence is a phenomenon with a longer or much longer delay in re radiation. Note that rays, x-rays, uv, visible light and ir radiations may all stimulate fluorescence.


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An advanced textbook/resources for experimental techniques?

... recent textbook that focuses on the current biology experimental techniques? Obviously, stuff on blots, but the more advanced stuff too on using fluorescence and radioactive labeling, site-directed mutagenesis, etc. In the exams we constantly have to design experiments and describe the "controls." ...

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by Dorothea
Sun Aug 23, 2009 1:00 pm
 
Forum: General Discussion
Topic: An advanced textbook/resources for experimental techniques?
Replies: 0
Views: 48

Photosynthesis

well you can monitor concentrations of different chemicals by spectrophotometric assays. you can monitor organelles by fluorescence microscopy etc. What exactly are you talking about? Your questions are so general that it is impossible to answer them.

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by MrMistery
Wed Aug 12, 2009 11:23 pm
 
Forum: Cell Biology
Topic: Photosynthesis
Replies: 10
Views: 267

How to measure cell pearmability/uptake in yeast?

Normalizing amount of dye or fluorescence is kinda difficult. You might have to do quite a few trials.

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by mith
Mon Feb 09, 2009 6:52 pm
 
Forum: Molecular Biology
Topic: How to measure cell pearmability/uptake in yeast?
Replies: 1
Views: 306

Enzyme-linked immunosorbent assay(ELISA)

... is linked to an enzyme, and in the final step a substance is added that the enzyme can convert to some detectable signal. Thus in the case of fluorescence ELISA, when light of the appropriate wavelength is shone upon the sample, any antigen/antibody complexes will fluoresce so that the amount ...

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by loyolite
Thu Jan 29, 2009 3:52 am
 
Forum: Biology Movies
Topic: Enzyme-linked immunosorbent assay(ELISA)
Replies: 1
Views: 1380

Why does chlorophyll always fluoresce in red?

Regardless of the color of the light, chlorophyll always fluoresce red light. I think I understand the basic principles of fluorescence, but I dont understand why it always is red light from chlorophyll. Is there any easy explanation to this that even I understand? Thanks

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by poobear
Wed Jan 28, 2009 7:00 pm
 
Forum: Botany Discussion
Topic: Why does chlorophyll always fluoresce in red?
Replies: 4
Views: 983
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