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Dictionary » F » Fluorescence

Fluorescence

Fluorescence

(Science: chemistry, physics) The emission of one or more photons by a molecule or atom activated by the absorption of a quantum of electro magnetic radiation.

Typically the emission, that is of longer wavelength than the excitatory radiation, occurs within 10exp 8 seconds: phosphorescence is a phenomenon with a longer or much longer delay in re radiation. Note that rays, x-rays, uv, visible light and ir radiations may all stimulate fluorescence.


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Re: Device for ratiometric fluorescence measurement

If you don't mind losing the flexibility of scanning the spectrum or rapidly changing ex/em bands, you would build a 2-band fluorescence colorimeter. You'd need two emission filters and one or two excitation filters, arranged around your cuvette so that the excitation and emission light ...

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by jonmoulton
Mon Mar 04, 2013 4:58 pm
 
Forum: General Discussion
Topic: Device for ratiometric fluorescence measurement
Replies: 1
Views: 61

Device for ratiometric fluorescence measurement

Hi， In the lab，I use fluorescence spectrometer for the ratiometric fluorescence measurement which needs to measure optical intensity at two different wavelenghts. However, the fluorescence spectrometer is expensive and huge. I am wondering ...

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by LittleMike
Mon Mar 04, 2013 3:40 pm
 
Forum: General Discussion
Topic: Device for ratiometric fluorescence measurement
Replies: 1
Views: 61

Difference between ICC and IF

I also have some doubts about fluorescence microscopy!? What technique we can use to visualise by fluorescence microscopy a transfected proteins that laks any fluorochrome in mammalian cell cultures? Does anyone have any ideas?

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by simina
Tue Feb 19, 2013 9:08 pm
 
Forum: Molecular Biology
Topic: Difference between ICC and IF
Replies: 7
Views: 7520

Two-color in situ help

Thanks for the reply. I looked at whole embryos and the auto-fluorescence was so weak that it wouldn't even be picked up by our most sensitive cameras. I also suspected the endogenous peroxidase activity but that did not seem to factor in either since ...

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by vastgenome
Thu Feb 14, 2013 11:59 pm
 
Forum: Molecular Biology
Topic: Two-color in situ help
Replies: 3
Views: 255

Why are genes regulated?

In this plasmid, another DNA sequence produce the GPF (green fluorescence protien) that results in the bacteria fluorescing under UN light. The there gene araC regulates the expression of GFP. Why are genes regulated in bacteria?

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by Remii
Tue Oct 30, 2012 10:01 am
 
Forum: General Discussion
Topic: Why are genes regulated?
Replies: 2
Views: 449
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