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... I work with 96-well plates. I use the Perkin Elmer TSA plus kit (cy3/Fluorescein) for two colors. Two mRNA probes (one is Digoxigenin-labeled ... hybridized at 65C overnight. I use anti-Digoxigenin-POD for the first antibody reaction, coupling it with cy3 (red) for the TSA reaction. Then ...
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