Fixative

Hi everyone, What should be the original concentration of ethanol or methanol used in the preparation of Carnoy's fixative solution? I know it is alcohol: glacial acetic acid( 3:1). But what is the original concentration of the alcohol? Thank you very much :)

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... if I understand what you want but, did you try simply change the water for organic solvent that will evaporate? Fix the fruit with protein linking fixative like 2% paraformaldehyde or glutaraldehyde for several days. Put the fixed fruit into serie of organic solvent concentration gradient- 30% ...

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Hello, Has anyone done an IHC experiment in Zebrafish embryos for detecting 8-oxoG? I was wondering what would be the best fixative to use? Thanks

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... you must use different concentrations and not just several times of the highest concentration. This probably has to do with high concentrations of fixative (e.g. 96% ethanol) damaging the sample by dissolving lipid membranes of the cells but if this is done gradually, no such damage takes place. ...

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PFA is just a fixative. Beware that PFA may cause autofluorescence and give you a lot of unwanted background staining. Triton X-100 is good for permeabilization. Adding some Tween 20 will help as well.

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