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Fixative

Fixative

1. Serving to fix, bind, or make firm or stable.

2. A substance used for the preservation of gross and histologic specimens of tissue, or individual cells, usually by denaturing and precipitating or cross-linking the protein constituents.

See: fluid, solution.


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Carnoy's fixation problem

Hi everyone, What should be the original concentration of ethanol or methanol used in the preparation of Carnoy's fixative solution? I know it is alcohol: glacial acetic acid( 3:1). But what is the original concentration of the alcohol? Thank you very much :)

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by Sue4
Thu Apr 25, 2013 7:37 pm
 
Forum: Cell Biology
Topic: Carnoy's fixation problem
Replies: 0
Views: 1053

Microorganisms and organic preservation?

... if I understand what you want but, did you try simply change the water for organic solvent that will evaporate? Fix the fruit with protein linking fixative like 2% paraformaldehyde or glutaraldehyde for several days. Put the fixed fruit into serie of organic solvent concentration gradient- 30% ...

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by triton
Wed Aug 22, 2012 7:01 pm
 
Forum: Microbiology
Topic: Microorganisms and organic preservation?
Replies: 1
Views: 3061

Queston about Zebrafish Whole mount Immunohistochemistry?

Hello, Has anyone done an IHC experiment in Zebrafish embryos for detecting 8-oxoG? I was wondering what would be the best fixative to use? Thanks

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by Vivkrish
Tue Apr 24, 2012 7:58 pm
 
Forum: Molecular Biology
Topic: Queston about Zebrafish Whole mount Immunohistochemistry?
Replies: 0
Views: 811

using ethanol 50% then 70%??

... you must use different concentrations and not just several times of the highest concentration. This probably has to do with high concentrations of fixative (e.g. 96% ethanol) damaging the sample by dissolving lipid membranes of the cells but if this is done gradually, no such damage takes place. ...

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by biohazard
Mon Nov 02, 2009 1:44 pm
 
Forum: Microbiology
Topic: using ethanol 50% then 70%??
Replies: 1
Views: 1658

Paraormaldehyde action in immunofluorescence.

PFA is just a fixative. Beware that PFA may cause autofluorescence and give you a lot of unwanted background staining. Triton X-100 is good for permeabilization. Adding some Tween 20 will help as well.

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by ab06
Fri Sep 07, 2007 6:35 pm
 
Forum: Cell Biology
Topic: Paraormaldehyde action in immunofluorescence.
Replies: 8
Views: 8686
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