Fitc

Thanks a lot for your quick reply and the suggestion on different possibility. The FITC-A histogram looks quite okey, there is only one sharp peak. So that`s why I wonder why there would be two peaks in FSC-A histogram. I will take your suggestion to use the 7-ADD ...

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How does that look on a graph = Is everything ok in FITC channel (histogram/dot plot/whatever you use)? I.e. does everything look ok there, or do you get two peaks on FITC as well? Maybe the main point here is: does it matter that you have two BT20 ...

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... the dead ones as well). Do you have any specific marker for BT20 that you can use to verify this? What was the antibody on the fluorochrome (FITC) you used, and how does that look on a graph?

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... I would like to assess the expression of cannabinoid receptor 2 on the surface of my cells by flow cytometry, but I can't find any antibodies, FITC conjugated against it. Does someone already used one? Thanks a lot

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... this peptide. They are not radioactive labeled. I can only detect them by Western blot. Another way is may detect them by FACS because we have FITC labeled peptides. But I'm not sure if I can do this by this way :) I will try freeze-thaw protocol, may this is the softer way for breaking the ...

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