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Fitc

Fitc --


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hybridization probe

... You can try this pair - Fluorescein (FITC)/ROX (carboxy-X-rhodamine).

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by Cat
Sun Sep 22, 2013 3:37 pm
 
Forum: Genetics
Topic: hybridization probe
Replies: 2
Views: 1082

Re: Lateral Flow Assay

... that I cannot tether this VEGF aptamer since it is only 28 NT long? A friend did IgE aptamer that was under 50 NT and got it to work (she used FITC with her antibodies though). Hopefully you are assessing the conjugation before you start the assay. Can you check how the tethering went?

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by jonmoulton
Thu Mar 08, 2012 6:55 pm
 
Forum: Cell Biology
Topic: Lateral Flow Assay
Replies: 1
Views: 1551

Lateral Flow Assay

... that I cannot tether this VEGF aptamer since it is only 28 NT long? A friend did IgE aptamer that was under 50 NT and got it to work (she used FITC with her antibodies though). If any of this is confusing or needs further clarification, please let me know! I am ultra confused by this point. ...

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by knclayton
Wed Mar 07, 2012 11:03 pm
 
Forum: Cell Biology
Topic: Lateral Flow Assay
Replies: 1
Views: 1551

FACS issue for Apoptosis?! Propidium Iodide Vs Annexin V-Cy5

... and analyze using FACS (BD FACS CANTO 2). Propidium Iodide Vs Annexin V - Cy5 1. I am using 2 step Annexin V detection (We dont have Annexin V-FITC). Will this work effectively compared to Annexin-FITC. (rabbit anti-Annexin V antibody for 10 min, WASH,Goat Anti-rabbit conjugated to Cy5 for ...

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by pjberlin
Thu May 13, 2010 2:34 pm
 
Forum: Molecular Biology
Topic: FACS issue for Apoptosis?! Propidium Iodide Vs Annexin V-Cy5
Replies: 0
Views: 2563

Enquiry about interpreting the FACS result

Thanks a lot for your quick reply and the suggestion on different possibility. The FITC-A histogram looks quite okey, there is only one sharp peak. So that`s why I wonder why there would be two peaks in FSC-A histogram. I will take your suggestion to use the 7-ADD ...

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by Crystalbin
Wed Feb 04, 2009 3:53 pm
 
Forum: Cell Biology
Topic: Enquiry about interpreting the FACS result
Replies: 4
Views: 4600
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