Home Forum Dictionary Articles Tutorials Books Directory Share your work


Edit page | Page history | Printable version

Dictionary » F » Fish analysis

Fish analysis

Definition

noun

An analysis based on the binding of a fluorescent probe (e.g. DNA or RNA probe) to detect and localize the presence of specific DNA sequences on chromosomes in cloned bacterial or cultured eukaryotic cells.


Supplement

The procedure of FISH involves the following steps: (1) Preparation of microscopic slide with cells in metaphase of mitosis (2) The slide is treated with a weak base to denature the DNA. (3) A radioactively labeled probe is poured onto the slide. (4) Expose slide to photographic emulsion for a few days or weeks. (5) Develop emulsion.


Word origin: Fish, an acronym for Fluorescence ‘’in situ’’ hybridization

See also: fluorescence microscopy


Please contribute to this project, if you have more information about this term feel free to edit this page



Results from our forum


Can anyone help Identify these fish vertebra?

I am doing a dietary analysis of the Eurasian Otter and I am currently struggling to identify some fish vertebra. Bones have been found in Otter spraints collected in Scottish fresh water environment. Can anyone ...

See entire post
by Ottermad83
Mon Mar 12, 2012 1:21 pm
 
Forum: Zoology Discussion
Topic: Can anyone help Identify these fish vertebra?
Replies: 1
Views: 1177

Re: Fish transfaunation from public to private waters - how to d

... of pollutants such as heavy metals are present in the first but absent in the latter. It still would require a costly and lenghty preparation and analysis but it should be feasible. ...in the Philippines it takes such a long process and a difficult one. Sometimes bacteria still comes a long and ...

See entire post
by DocWillow
Fri Nov 25, 2011 7:38 am
 
Forum: Zoology Discussion
Topic: Fish transfaunation from public to private waters - how to d
Replies: 5
Views: 2606

Prevalence of gene

... to have a guide if you do this work. PSI and PHI BLASTS allow greater analysis based on repeated iterations based on the previous analysis (including ... sequenced now in most organisms. In my example, I have just found a fish match to the CD40 gene - most interesting ! From the data I have discussed ...

See entire post
by merv
Sun Oct 02, 2011 9:01 pm
 
Forum: Bioinformatics
Topic: Prevalence of gene
Replies: 8
Views: 7199

Fish transfaunation from public to private waters - how to d

... location. I've been thinking about possible solutions to determine if a fish caught in a private pond comes from a fish farm or from public waters. ... the latter. It still would require a costly and lenghty preparation and analysis but it should be feasible. I found a reference from a private ...

See entire post
by VascoResando
Fri Nov 26, 2010 2:39 pm
 
Forum: Zoology Discussion
Topic: Fish transfaunation from public to private waters - how to d
Replies: 5
Views: 2606

Calcium ions in the Cell

... same kind of yeast cells we used in our discussion of the two-hybrid analysis, and these cells are then treated with calcimycin, what organelle ... (2 are plasmid markers, the third is a result of activation of the fish-bait gene activation). I don't think this is what he meant, moreso that ...

See entire post
by someguy456345
Sat Apr 24, 2010 3:05 pm
 
Forum: Cell Biology
Topic: Calcium ions in the Cell
Replies: 4
Views: 5139
View all matching forum results

This page was last modified 09:20, 18 March 2009. This page has been accessed 12,061 times. 
What links here | Related changes | Permanent link