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Fingerprinting

Fingerprinting

The basic principle of the technique is to digest a large molecule with a sequence specific hydrolase to produce moderate size fragments that can then be run on an electrophoresis gel. Provided the hydrolase only cleaves at specific sites (e.g. Between particular amino acids or bases) then the fragments should be characteristic of that molecule. The technique can be used to distinguish strains of virus or to differentiate between similar but nonidentical proteins (peptide mapping). Not to be confused with footprinting.


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I have got some BIG issues! forgive the long post

... like domains (2 and 2) forming the membrane proximal region. The class II proteins appear to form (super) dimers at the cell surface. FINGERPRINTING AND DNA PROFILING Like the fingerprints that came into use by detectives and police labs during the 1930s, each person has a unique DNA ...

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by saddy51
Sun Jan 04, 2015 10:39 am
 
Forum: Genetics
Topic: I have got some BIG issues! forgive the long post
Replies: 1
Views: 1769

how many dna molecules in a cell? are they all the same?

... me a lot. And one more question. :) (I promise it is also the last one) Since DNA molecules in a human cell are different, how do forensics do DNA fingerprinting test? I mean, if they find one DNA molecule, which is from the murder's chromosome-1, in the crime scene, will they have to compare it ...

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by julianawhite
Tue Oct 30, 2012 2:05 pm
 
Forum: Cell Biology
Topic: how many dna molecules in a cell? are they all the same?
Replies: 5
Views: 6410

how many dna molecules in a cell? are they all the same?

... cell? are they all the same? if they are all the same, why different chromosomes have different number of genes? if they are not, how does dna fingerprinting make sure two different dna, which are from different chromosomes, from one person? Thanks in advance.

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by julianawhite
Mon Oct 29, 2012 3:34 pm
 
Forum: Cell Biology
Topic: how many dna molecules in a cell? are they all the same?
Replies: 5
Views: 6410

DNA question help

i am doing DNA fingerprinting assignment and i got stuck on this question if a restriction is "denatured", and therefore does not work, how would you recognize this on the gel?

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by ekfsksk
Sun Apr 01, 2012 10:04 pm
 
Forum: Molecular Biology
Topic: DNA question help
Replies: 3
Views: 1671

restriction enzymes in dna fingerprinting

In DNA comparisons between two people, which genes or sequences are chosen to compare? You can't compare all the chromosomes, because there are 3 billion base pairs. So which restriction enzymes are used, which sections of dna are chosen for comparison , how are they selected out, and how are the ge...

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by mattw
Fri Nov 19, 2010 3:59 pm
 
Forum: Genetics
Topic: restriction enzymes in dna fingerprinting
Replies: 1
Views: 2265
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