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Fingerprinting

Fingerprinting

The basic principle of the technique is to digest a large molecule with a sequence specific hydrolase to produce moderate size fragments that can then be run on an electrophoresis gel. Provided the hydrolase only cleaves at specific sites (e.g. Between particular amino acids or bases) then the fragments should be characteristic of that molecule. The technique can be used to distinguish strains of virus or to differentiate between similar but nonidentical proteins (peptide mapping). Not to be confused with footprinting.


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how many dna molecules in a cell? are they all the same?

... me a lot. And one more question. :) (I promise it is also the last one) Since DNA molecules in a human cell are different, how do forensics do DNA fingerprinting test? I mean, if they find one DNA molecule, which is from the murder's chromosome-1, in the crime scene, will they have to compare it ...

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by julianawhite
Tue Oct 30, 2012 2:05 pm
 
Forum: Cell Biology
Topic: how many dna molecules in a cell? are they all the same?
Replies: 5
Views: 3544

how many dna molecules in a cell? are they all the same?

... cell? are they all the same? if they are all the same, why different chromosomes have different number of genes? if they are not, how does dna fingerprinting make sure two different dna, which are from different chromosomes, from one person? Thanks in advance.

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by julianawhite
Mon Oct 29, 2012 3:34 pm
 
Forum: Cell Biology
Topic: how many dna molecules in a cell? are they all the same?
Replies: 5
Views: 3544

DNA question help

i am doing DNA fingerprinting assignment and i got stuck on this question if a restriction is "denatured", and therefore does not work, how would you recognize this on the gel?

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by ekfsksk
Sun Apr 01, 2012 10:04 pm
 
Forum: Molecular Biology
Topic: DNA question help
Replies: 3
Views: 1151

restriction enzymes in dna fingerprinting

In DNA comparisons between two people, which genes or sequences are chosen to compare? You can't compare all the chromosomes, because there are 3 billion base pairs. So which restriction enzymes are used, which sections of dna are chosen for comparison , how are they selected out, and how are the ge...

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by mattw
Fri Nov 19, 2010 3:59 pm
 
Forum: Genetics
Topic: restriction enzymes in dna fingerprinting
Replies: 1
Views: 1880

questions about DNA fingerprinting

In DNA fingerprinting, when u amplify the STRs by PCR, how how do you control the elongation of the primer so that the elongation stops exactly at the end of the STR?

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by tracyliu
Thu Oct 21, 2010 11:09 am
 
Forum: Genetics
Topic: questions about DNA fingerprinting
Replies: 1
Views: 1719
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