Filtrate

... of the test tube into the filter funnel. 8. Wash any trapped protoplasts through the filter with 10cm3 of 13% sorbitol solution. Collect all the filtrate in a centrifuge tube. 9. Balance the centrifuge tubes then spin for roughly 5 minutes at 2,000 rpm. <-- does it have to be exactly 2,000 ...

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... the neprhon. (Which would cause dehydration) Anyway, hope this isn't wrong, but as I said, best wait 'til someone confirms it :) Edit: Oh, and the filtrate becomes more and more concentrated due to the removal of water and such. In theory, all that will remain is urea and salt, and other waste ...

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... I'm new to this forum but a friend told me about how great you guys are :) Any ways on to my question.. I am having trouble understanding how the filtrate becomes more concentrated when it goes through the nephron... I don't really understand the process :? Here's the actual question: Why does ...

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Since proteins and blood cells are way too big to be filtraded in the glomerulus, yes, they are not part of the filtrate. But don't forget that due to some diseases, there may be blood cells in the filtrate (and in the urine, too). Also, when someone's got diabetes, there's glucose ...

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... non-filterable particles in the blood. The proteins also hold onto more water, so fewer particles and less water goes through, meaning less total filtrate. There may be other processes at work here that also contribute...

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