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Feeder layer

Feeder layer

(Science: cell culture) in order to culture some cells, particularly at low or clonal density, it is necessary to use a layer of less fastidious cells to condition the medium.

Often the cells of the feeder layer are irradiated or otherwise treated so that they will not proliferate. In some cases the feeder layer may be producing growth factors or cytokines.


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Re: Can I culture HESCs without feeder cell layer?

... tried hESC's though. I haven't used them personally (I still use a feeder cell layer), but you might try contacting them. I did notice on their site that they've had success ...

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by biofrog
Sun Aug 24, 2008 11:49 pm
 
Forum: Cell Biology
Topic: Can I culture HESCs without feeder cell layer?
Replies: 1
Views: 1999

Can I culture HESCs without feeder cell layer?

... ). I wonder if I can culture them without the feeder cell layer. You are very appreciated if you can let me know the protocol, or share me your experience. ...

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by pguo
Wed May 14, 2008 7:56 pm
 
Forum: Cell Biology
Topic: Can I culture HESCs without feeder cell layer?
Replies: 1
Views: 1999

Looking for: Protocol to Culture HESCs without Feeder

... stem cell bank). Right now, I am co-culturing the cell lines with feeder cell line MEF (from ATCC). I wonder if anyone has the experience to culture H1 and/or H9 without using feeder layer. You are very appreciated if you can let me know the protocol. Thank you.

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by biolearner
Mon May 12, 2008 8:31 pm
 
Forum: Cell Biology
Topic: Looking for: Protocol to Culture HESCs without Feeder
Replies: 0
Views: 1008


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