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Fcs

Fcs --> foetal calf serum

(Science: cell culture) The serum originating from embryonic calves and an expensive component of standard culture media for many types of animal tissue cells.

It is used as an important part of growth media for promoting the growth of tissue cultures. It provides a source of growth hormones and other growth factors.


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Why dialyse DMEM before protein purification (Ni-Affinity)?

... a dialysis?" We transfected HEK293T in order to express a his-tagged protein. The cells were kept in DMEM (high glucose, modified with 5% FCS and 1% streptomycin/penicillin), which was collected daily and exchanged with fresh modified DMEM. The collected supernatants where then dialysed ...

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by LittleBird
Sun May 20, 2012 3:43 pm
 
Forum: Molecular Biology
Topic: Why dialyse DMEM before protein purification (Ni-Affinity)?
Replies: 5
Views: 2040

What does this mean??? Please help

... ("Cr-labeled ; 30 min, 100 juCi/ml) were incubated with Ly antiserum diluted in phosphate-buffered saline with 5% fetal calf serum (PBS-FCS) for 1/2 h at 37°C, washed once, brought up in 1 ml of freshly thawed rabbit serum (diluted 1 :8 in PBS), and incubated for a further 1/2 h at 37°C ...

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by speakers2
Sun Oct 16, 2011 1:06 am
 
Forum: General Discussion
Topic: What does this mean??? Please help
Replies: 0
Views: 1648

Problems with C2C12 resuscitation

... 500mL Pyruvate Na: Gibco (11360-039) 5mL NEAA: Gibco (11140-035) 5mL Pen Strep: Hyclone Thermo (SV30010) 1mL Fungizone: Gibco (15290-018) 0,5mL FCS: Sigma (F2442) 50mL L-Glutamine: Sigma (G7513) 5mL I really hope someone could help me. Thank you very much in advance for your help. Cécile

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by CecileL
Wed Jun 23, 2010 7:50 am
 
Forum: Cell Biology
Topic: Problems with C2C12 resuscitation
Replies: 1
Views: 1819

Re: Do someone know SH-SY5Y cells??

... with polylysinated dishes? with this simple method I solved all my problem of non attached cells. As Medium it is suggested to use F12:MEM 1:1, FCS(or FBS) 10%, Pen/Strep 2%, Glutamin 200mM, Na-pir 100mM. Hope to have been useful, have a good work!!

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by violsk
Wed Feb 24, 2010 6:53 am
 
Forum: Cell Biology
Topic: Do someone know SH-SY5Y cells??
Replies: 2
Views: 2169

cell culture incubator

... 6.5% CO2 or 10% CO2. I know they are not doing it because that is how they get the desired real 5%. Interesting.... 2. I mean when one uses 10% FCS to culture a given lell line, and after transfection one increases the serum content in the culture medium to 20% FCS saying transfected cells did ...

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by kk
Mon Sep 28, 2009 8:50 am
 
Forum: Cell Biology
Topic: cell culture incubator
Replies: 2
Views: 1580
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