Fcs

... a dialysis?" We transfected HEK293T in order to express a his-tagged protein. The cells were kept in DMEM (high glucose, modified with 5% FCS and 1% streptomycin/penicillin), which was collected daily and exchanged with fresh modified DMEM. The collected supernatants where then dialysed ...

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... ("Cr-labeled ; 30 min, 100 juCi/ml) were incubated with Ly antiserum diluted in phosphate-buffered saline with 5% fetal calf serum (PBS-FCS) for 1/2 h at 37°C, washed once, brought up in 1 ml of freshly thawed rabbit serum (diluted 1 :8 in PBS), and incubated for a further 1/2 h at 37°C ...

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... 500mL Pyruvate Na: Gibco (11360-039) 5mL NEAA: Gibco (11140-035) 5mL Pen Strep: Hyclone Thermo (SV30010) 1mL Fungizone: Gibco (15290-018) 0,5mL FCS: Sigma (F2442) 50mL L-Glutamine: Sigma (G7513) 5mL I really hope someone could help me. Thank you very much in advance for your help. Cécile

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... with polylysinated dishes? with this simple method I solved all my problem of non attached cells. As Medium it is suggested to use F12:MEM 1:1, FCS(or FBS) 10%, Pen/Strep 2%, Glutamin 200mM, Na-pir 100mM. Hope to have been useful, have a good work!!

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... 6.5% CO2 or 10% CO2. I know they are not doing it because that is how they get the desired real 5%. Interesting.... 2. I mean when one uses 10% FCS to culture a given lell line, and after transfection one increases the serum content in the culture medium to 20% FCS saying transfected cells did ...

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