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Fcs

Fcs --> foetal calf serum

(Science: cell culture) The serum originating from embryonic calves and an expensive component of standard culture media for many types of animal tissue cells.

It is used as an important part of growth media for promoting the growth of tissue cultures. It provides a source of growth hormones and other growth factors.


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Propagation and Transfection of BHK cells?

... efficiency in hard to transfect cells like kidney fibroblasts as well as in standard cells, you can work with complete media (including FCS) without changing media, high reproducibility because Viromers form stable particles of 300-400nm in buffer. If you are interested I may send a ...

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by OliviaZ
Tue Apr 08, 2014 10:48 am
 
Forum: Molecular Biology
Topic: Propagation and Transfection of BHK cells?
Replies: 2
Views: 1385

Overexpression

... efficiency in hard to transfect cells like Primary macrophages as well as in standard cells, you can work with complete media (including FCS) without changing media, high reproducibility because Viromers form stable particles of 300-400nm in buffer. If you are interested I may send a ...

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by OliviaZ
Tue Apr 08, 2014 10:45 am
 
Forum: Molecular Biology
Topic: Overexpression
Replies: 2
Views: 1633

Why dialyse DMEM before protein purification (Ni-Affinity)?

... a dialysis?" We transfected HEK293T in order to express a his-tagged protein. The cells were kept in DMEM (high glucose, modified with 5% FCS and 1% streptomycin/penicillin), which was collected daily and exchanged with fresh modified DMEM. The collected supernatants where then dialysed ...

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by LittleBird
Sun May 20, 2012 3:43 pm
 
Forum: Molecular Biology
Topic: Why dialyse DMEM before protein purification (Ni-Affinity)?
Replies: 5
Views: 2679

What does this mean??? Please help

... ("Cr-labeled ; 30 min, 100 juCi/ml) were incubated with Ly antiserum diluted in phosphate-buffered saline with 5% fetal calf serum (PBS-FCS) for 1/2 h at 37°C, washed once, brought up in 1 ml of freshly thawed rabbit serum (diluted 1 :8 in PBS), and incubated for a further 1/2 h at 37°C ...

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by speakers2
Sun Oct 16, 2011 1:06 am
 
Forum: General Discussion
Topic: What does this mean??? Please help
Replies: 0
Views: 2114

Problems with C2C12 resuscitation

... 500mL Pyruvate Na: Gibco (11360-039) 5mL NEAA: Gibco (11140-035) 5mL Pen Strep: Hyclone Thermo (SV30010) 1mL Fungizone: Gibco (15290-018) 0,5mL FCS: Sigma (F2442) 50mL L-Glutamine: Sigma (G7513) 5mL I really hope someone could help me. Thank you very much in advance for your help. Cécile

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by CecileL
Wed Jun 23, 2010 7:50 am
 
Forum: Cell Biology
Topic: Problems with C2C12 resuscitation
Replies: 1
Views: 1984
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