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Far western analysis

Far western analysis

yeast gene, induced by factor, that causes cells to arrest in G1 phase, by interacting with the G1 cyclin, CLN2.


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Can you recommend a vector?

Hi all, I am doing some mutation analysis. I have generated 4 mutants and a wild type ... We can maintain expression (as detected by western and qPCR) of the transgene for 8-12 cell passages, ... line which are apparently notorious for this. So far i am looking for a human promoter within the plasmid ...

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by JimmyTee
Wed Jun 15, 2011 3:17 pm
 
Forum: Molecular Biology
Topic: Can you recommend a vector?
Replies: 0
Views: 1452

Scholarly article help

Figure 2C: What is a western blot? What is it supposed to show? They are quite clear what is in what lane in the legend: Western blot analysis showing the presence and absence of CdhA expression in the whole lysates of mutant, wild-type, ...

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by canalon
Thu Mar 10, 2011 8:16 pm
 
Forum: Microbiology
Topic: Scholarly article help
Replies: 1
Views: 1191

Re: Brain size=IQ level theory (Blacks vs Whites & Asians)

... [Lynn, R., (2006). Race differences in intelligence: An evolutionary analysis. Augusta, GA: Washington Summit Publishers.] and Lynn and Vanhanen ... studies are needed to address the issue of measurement bias of western IQ tests for Africans. Keywords: Group differences; Black-White differences; ...

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by TruthIsHoly
Fri Jun 25, 2010 6:44 pm
 
Forum: Human Biology
Topic: Brain size=IQ level theory (Blacks vs Whites & Asians)
Replies: 72
Views: 270168

Recombination in RNA viruses,Ruzic,Kovac

... of poliovirus could be mapped by recombination analysis ± recombination was not thought to be a ... compensate for these effects. For example, the Western equine encephalitis (WEE) complex viruses ... of a ` true ' phylogenetic taxonomy. Far from being an incidental process best ignored ...

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by anakovacevic
Sat Jun 12, 2010 8:28 pm
 
Forum: Cell Biology
Topic: Recombination in RNA viruses,Ruzic,Kovac
Replies: 2
Views: 36082

Western Blotting protocol using BioRad

Hi every one I am new to protein analysis field and want some help. I bought all reagents from BioRad required for western blotting. I need a step by step protocol (in detail) please to start runnig this assay. Can any ...

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by roniadam
Mon Mar 15, 2010 1:23 pm
 
Forum: Molecular Biology
Topic: Western Blotting protocol using BioRad
Replies: 2
Views: 1542
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