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Factor ix assay

Factor ix assay

a test used to measure the activity of a blood clotting factor IX (christmas factor). This test may be used to evaluate excessive bleeding. Abnormally low factor IX assays may be seen in the following conditions: congenital deficiency of factor IX, fat malabsorption, heparin administration, cirrhosis, vitamin k deficiency and warfarin administration.

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mRNA vs. cell reporter assay

Hi, I have a GFP reporter cell line that drives GFP expression upon activation of a transcription factor. In theory, I can study transcription factor activation via the GFP signal, or by measuring the mRNA of the transcription factor. I wonder what are the pros and cons ...

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by aguy
Mon Jul 07, 2014 2:50 am
Forum: Cell Biology
Topic: mRNA vs. cell reporter assay
Replies: 2
Views: 2930

Direct or indirect protein-DNA interaction

... would one go about deducing whether a certain DNA binding transcription factor has a direct interaction with a gene of interest ( we know that the ... region of the gene of interest. I'm trying to think of ways to assay this, but I can't come up with anything useful. Perhaps some kind of ...

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by nukeman
Wed Oct 31, 2012 5:21 pm
Forum: Molecular Biology
Topic: Direct or indirect protein-DNA interaction
Replies: 1
Views: 1364

Transcription Factor Question

For how to determine the transcription factor binding sites in the whole genome, I'd use ChIP with ChIP sequencing. I'm ... Monday. As for the for the first question, I guess use a DNAse1 Foot-Printing Assay? A classmate of mine mentioned knockout mutations but, it's not asking what ...

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by mxnick262
Tue Apr 17, 2012 3:47 pm
Forum: Molecular Biology
Topic: Transcription Factor Question
Replies: 7
Views: 3035

enzyme inhibition assay

... some help to interpret my results...I'm working with anthrax lethal factor, which is a Zn2+ dependent metalloproteinase. I would like to calculate Ki of an inhibitor using a FRET inhibition assay.The FRET assay are run for 30 minutes at 25 °C. At the end I obtain a graph ...

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by claire84
Thu Jan 12, 2012 11:15 am
Forum: Molecular Biology
Topic: enzyme inhibition assay
Replies: 0
Views: 1280

Laboratory methods

... Here's the reference: Maiso, P. et al. 2007. The insulin-like growth factor-I receptor inhibitor NVP-AEW541 provokes cell cycle arrest and apoptosis ... (patchy) interpretation: Cell proliferation, cell cycle and apoptosis assays- They are analysing the normal growth of the MM cells as a control ...

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by lrob
Sun Mar 06, 2011 1:44 pm
Forum: Molecular Biology
Topic: Laboratory methods
Replies: 1
Views: 2553
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