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Facs

Facs

(Science: abbreviation)

1. Fluorescence-activated cell sorter.

2. Fellow of the American College of surgeons.


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FACS Cells Markers

Hi everyone, I am looking for some informations about immune cells markers in mouse. I founded some markers, but I'm not sure if they are ok. So can you tell me what do you think about my selection ? LT : CD3 LB : CD19 Neutrophiles (most important cells for me) : Ly6G high and ? Dendritic cells : CD...

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by aureliegineste
Wed Nov 20, 2013 10:26 am
 
Forum: Cell Biology
Topic: FACS Cells Markers
Replies: 0
Views: 407

Re: ChIP-Seq for small number of cells

Hi there, I would like to perform a ChIP-seq on primary cells (FACS sorted). However, I can get only a limited number of cells per mouse (~3000/mouse). So I'd end up with 20,000 cells, is that enough for ChIP-seq? How about reproducibility? I read the paper ...

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by fizza9
Thu Aug 18, 2011 11:54 am
 
Forum: Genetics
Topic: ChIP-Seq for small number of cells
Replies: 4
Views: 3869

Re: FACS and growth curve result

While studying the growth curve one should also be very aware about the different terminologies which are actually being employed. These may be Idiophase, trophophase. One should also consider the concept of biphasic growth which is also kknown as di auxic growth.

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by mohit
Sat Mar 26, 2011 5:09 am
 
Forum: Cell Biology
Topic: FACS and growth curve result
Replies: 4
Views: 1541

FACS and growth curve result

... which express GFP. I studied the cell growth under various conditions and observed the number of cells are growing more in some cases. Then I did FACS. But I observed FACS result is same for all cases. Do FACS result has to corroborate growth curve? I thought FACS gives the % of cells expressing ...

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by nusrat1977
Fri Mar 25, 2011 1:39 am
 
Forum: Cell Biology
Topic: FACS and growth curve result
Replies: 4
Views: 1541

Re:

... sections) and went through the tutorial and found that the percentage of G0 cells could be calculated using FLUORESCENCE ACTIVATED CELL SORTER (FACS)

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by mjomha
Tue Mar 08, 2011 7:26 pm
 
Forum: Molecular Biology
Topic: HELP! with ethidium bromide experiment
Replies: 15
Views: 8011
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