Facs

Hi there, I would like to perform a ChIP-seq on primary cells (FACS sorted). However, I can get only a limited number of cells per mouse (~3000/mouse). So I'd end up with 20,000 cells, is that enough for ChIP-seq? How about reproducibility? I read the paper ...

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While studying the growth curve one should also be very aware about the different terminologies which are actually being employed. These may be Idiophase, trophophase. One should also consider the concept of biphasic growth which is also kknown as di auxic growth.

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... which express GFP. I studied the cell growth under various conditions and observed the number of cells are growing more in some cases. Then I did FACS. But I observed FACS result is same for all cases. Do FACS result has to corroborate growth curve? I thought FACS gives the % of cells expressing ...

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... sections) and went through the tutorial and found that the percentage of G0 cells could be calculated using FLUORESCENCE ACTIVATED CELL SORTER (FACS)

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FACS and live/dead cell fluorescent dyes (like propidium iodide) It is quick and easy if you have acess to this kind of machine. Otherwise no idea.

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