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(Science: immunology) fragment of immunoglobulin prepared by papain treatment. Fab fragments (45 kD) consist of one light chain linked through a disulphide bond to a portion of the heavy chain and contain one antigen binding Site. They can be considered as univalent antibodies.

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Protein bonding(antigens)

... is not covalent. It has to do with chemical noncovalent bonding/binding. Here is a picture that shows the "pocket" of the antibody Fab fragment with an antigen (epitope): http://www.pnas.org/content/102/14/4984/F3.large.jpg and a space filling model: http://www.google.com/imgres?imgurl=http://www.scripps.edu/newsandviews/e_20050228/enlarge1.jpg&imgrefurl=http://www.scripps.edu/newsandviews/e_20050228/enlarge.html&usg=__zru4TKFYtwhCQl1ThS6a-gxA5ZY=&h=403&w=324&sz=26&hl=en&start=105&sig2=eAIM5QTonqwSAsvCSkBYJQ&um=1&itbs=1&tbnid=26BBr_-5Z4oDPM:&tbnh=124&tbnw=100&prev=/images%3Fq%3Dantigen%2Bbinding%26start%3D84%26um%3D1%26hl%3Den%26safe%3Dactive%26sa%3DN%26rlz%3D1T4GGLL_enUS309US312%26ndsp%3D21%26tbs%3Disch:1&ei=YwP8S-qFKZvsNcC3jDk

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by kolean
Tue May 25, 2010 5:07 pm
Forum: Molecular Biology
Topic: Protein bonding(antigens)
Replies: 8
Views: 10098

Fab library constructing-ligation/transformation problem

I am working on constructing a phage library to express antibodies and currently I am doing the Fab library constructing work. I did PCR and got light chain (LC) products. There is a LC stuffer in the vector so I digested and gel purified the digested phagemid vector and ...

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by FABphage
Tue Jan 19, 2010 9:54 pm
Forum: Molecular Biology
Topic: Fab library constructing-ligation/transformation problem
Replies: 0
Views: 1201

Fully synthetic phage antibody libraries

I have a question ,that the fully synthetic phage antibody libraries was established for scFv,could Fab can use this method?thanks :D

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by jeffliyong
Mon Dec 28, 2009 5:15 am
Forum: Human Biology
Topic: Fully synthetic phage antibody libraries
Replies: 0
Views: 1012

Arent I too young to have high blood pressure?!

... family for 2 generations has had this problem! how the heck... if you guys could let me know what to eat and not eat and other stuff that would be fab...

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by Twila
Thu Apr 30, 2009 1:21 pm
Forum: General Discussion
Topic: Arent I too young to have high blood pressure?!
Replies: 4
Views: 4192


... 2 different light chains I think it is 2 identical heavy and 2 indentical light? 2) Cleavage of IgG by papain produces which of the following: A Fab fragment Monovalent antigen binding fragments Isolated heavy chains F(ab')2 Isolated light chains I think it could be a fab fragment but not sure! ...

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by pinkypig
Thu Feb 05, 2009 5:12 pm
Forum: General Discussion
Replies: 0
Views: 3133
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