F plasmid

Hi guys, I'm a new guy in molecular biology. I plan to express a scfv in S2 cell using the plasmid pMT/bip/V5-His A (with enzyme site BglII and XbaI). Because there's a Bip signal sequence ahead of the enzyme site BglII, so I CAN NOT add Kozak sequence in the N-Term ...

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Hello, I have a related question. I try to ligate annealed oligos into double digested (different enzymes) plasmid. The oligos I annealed were not modified with phosphates. Do I need to phosphorylate or dephosphorylate any of the two? As far as I understand, it is not necessary ...

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... the gene ABC at the position XYZ of the 1st chromosomes with a new gene so it can production for new protein instead of old protein. After have a plasmid that included the targeting gene to transfer, how the gene to be inject can detect the position (which chromosome, which position) to injecting. ...

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I'm having difficulty understanding how exactly a plasmid does what it does. I know that it's generally located in bacteria, and it's non-essential to them. I don't understand how they replicate though. Does it work the same way as a bacterium's chromosomal ...

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... a gene within the same region (operon), and the issue (and difference between the two) are not so obvious for us. Our vector is a circular plasmid. Can you elaborate on what you mean by two linear pieces that are homologous to each other? Do you mean using a linear DNA to KO the gene of ...

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