Dictionary » F » FISH

FISH

Definition

An abbreviation for fluorescence ‘’in situ’’ hybridization, a cytogenetic technique using fluorescent probe to detect and localize the presence of specific DNA sequence on chromosomes.


Supplement

FISH can be used to find specific features in DNA for use in genetic counseling, medicine, and species identification. FISH can also be used to detect and localize specific mRNAs within tissue samples.


Word origin: from the abbreviation of fluorescence in situ hybridization.


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FISH and Taqman probes

I want to quantify bacteria , and this bacteria has been detected by using FISH probe(targets 16S). Can I use this FISH probe as my TaqMan probe and design the forward and reverse primers on the two sides of it?

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by 2444256703
Tue Mar 03, 2015 6:28 am
 
Forum: General Discussion
Topic: FISH and Taqman probes
Replies: 0
Views: 108

Taqman probe design using FISH probe (need help)

Hi: Although they are both called "probes", FISH and Taqman probes have different characteristics. Probes for FISH need to be at least 1 kb long, to hybridize and "highlight" the target chromosomal DNA (metaphase chromosomes or interphase ...

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by protoemics
Wed Feb 04, 2015 8:11 am
 
Forum: Bioinformatics
Topic: Taqman probe design using FISH probe (need help)
Replies: 2
Views: 534

Taqman probe design using FISH probe (need help)

... and I need your help please. I want to quantify bacteria by using RT-PCR (Taqman), this bacteria has been detected (in previous study) by using FISH probe (targets 16S). Can I use this FISH probe (from the paper) as my TaqMan probe and design the forward and reverse primers on the two sides ...

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by biomart
Wed Feb 04, 2015 7:10 am
 
Forum: Bioinformatics
Topic: Taqman probe design using FISH probe (need help)
Replies: 2
Views: 534

Locomotory Evolution

There are advantages to both types of body symmetry. a bilaterian fish enjoys much faster, unidirectional movement compared to a jellyfish. This could be advantages if the bilaterian is a predator or a prey for example. in the cambrain explosion it is believed ...

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by Youngji
Sun Feb 01, 2015 12:36 pm
 
Forum: Evolution
Topic: Locomotory Evolution
Replies: 1
Views: 1494

Basic Knowledge about DNA Extraction

... and genetic disorders. Many techniques in biological research are involved with the extraction of DNA, such as Fluorescence In Situ Hybridization (FISH), a molecular technique used to identify and enumerate specific bacterial groups and Terminal Restriction Fragment Length Polymorphism (T-RFLP), ...

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by 2444256703
Thu Jan 22, 2015 8:56 am
 
Forum: Genetics
Topic: Basic Knowledge about DNA Extraction
Replies: 0
Views: 748
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