F2 fragment

... pombe cdc2, and S. cerevisiae cdc28 genes. Degenerate oligonucleotide PCR primers corresponding to the boxed amino acids were generated and a DNA fragment of the expected size was synthesized by PCR using these primers and maize cDNA library as a template. The PCR product was then used as a probe ...

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... difficult due to the high amount of splicing intermediates. I have so far used varying primers on the cDNA of arabidopsis in order to get some fragments that I could then ligate together. I have used proofreading polymerase, Taq, phusion, abgene, and I have then tried (and failed) to isolate, ...

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... SstI, EcoRI and HindIII located at the following positions: EcoRI position 42 SstI position 218 HindIII positions 328 and 5518 An EcoRI/SstI fragment (1350 bp long), containing the chicken virus X ns5 gene, was cloned into the vector, which had also been cut with PstI and EcoRI. (The chicken ...

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Hi all, I am prepare in entrance exam. I need help. A restriction endonuclease has the recognition sequence G/AATTC, where "/" indicate the cut side. This sequence is found, on average, once every 'X' residues in a chromosome. 'X' = Options a. 146 base - pairs b. 200 base - pairs c 256 bas...

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The number of fragment of each size depends on probability. The shorter ones are the most common. There is one fluorophore per fragment. So the the very numerous short fragment are very bright, to the point that they can be hard ...

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