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F2 fragment

F2 fragment

(Science: immunology) a fragment of an antibody protein which includes the antigen-binding portions but not the fc section. They can be produced by treating whole antibodies with proteases that will specifically cleave off the fc section.


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Souther blot and restriction mapping

... I would greatly appreciate any help with constructing a restriction map from Southern Hybridisation data. I can construct restriction maps from fragment data obtained through endonuclease digestion and separation by standard gel electrophoresis, however, Southern Blot data only shows those fragments ...

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by Mistersnuffle
Sat Oct 04, 2014 11:17 am
 
Forum: Genetics
Topic: Souther blot and restriction mapping
Replies: 0
Views: 18

Re: microsatellite instability

OK, another question: I can see how insertion/deletion can be detected by fragment analysis, but incorporation of a wrong nucleotide wouldn't cause change in the length of amplicon, so can that also be detected by fragment analysis?

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by kk
Thu Jul 10, 2014 2:45 pm
 
Forum: Molecular Biology
Topic: microsatellite instability
Replies: 3
Views: 2518

No "beginning-replication problem" on leading strand?

... those a continuous strand is produced until meeting replicating fork from another direction or end of the strand. The primer is removed by Okazaki fragment going in the other direction.

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by JackBean
Tue May 13, 2014 4:55 pm
 
Forum: Molecular Biology
Topic: No "beginning-replication problem" on leading strand?
Replies: 1
Views: 897

No "beginning-replication problem" on leading strand?

... is always so that we can replicate the end of the lagging strand. This is because the extreme end have a RNA primer to make the last Okazaki fragment, and after the removal of this primer it needs to somehow be replaced with DNA. I have the latest version of Molecular Biology of the Gene ...

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by poobear
Fri Apr 11, 2014 9:40 pm
 
Forum: Molecular Biology
Topic: No "beginning-replication problem" on leading strand?
Replies: 1
Views: 897

Gel electrophoresis

Hey guys! Would anyone know, in each of the Lane 2's, why there is a very large bright fragment that doesn't really confine to the shape of a rectangle? Just a general description would be helpful. Thank you!

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by downingrock
Wed Mar 12, 2014 12:44 pm
 
Forum: Genetics
Topic: Gel electrophoresis
Replies: 3
Views: 1764
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