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Extracts

Extract

(Science: pharmacology) a concentrated preparation of a vegetable or animal drug obtained by removing the active constituents therefrom with a suitable menstruum, evaporating all or nearly all the solvent and adjusting the residual mass or powder to a prescribed standard. Extracts are prepared in three forms, semiliquid or of syrupy consistency, pilular or solid and as dry powder.

Origin: L. Extractum


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cAMP 2nd Messenger Pathway and Experiments

... have adenylyl cyclase. The following experiments are conducted: Experiment 1: Cell membranes from cyc- cells were isolated and mixed with protein extracts from cells which exhibited adenylyl cyclase activity (cyc+ cells). Stimulating hormone, ATP, GTP, and Mg2+ were included in the mixture. The ...

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by mkay28
Tue Sep 24, 2013 10:43 pm
 
Forum: Cell Biology
Topic: cAMP 2nd Messenger Pathway and Experiments
Replies: 2
Views: 2249

Western Blot

... possible. One of the first techniques that can be used is to separate it according to how big it is, otherwise known as its molecular weight. When extracts are prepared from a cell, detergents can be used to make the proteins unfold into the linear chain of amino acids. Using a detergent such as ...

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by MarkHolland
Sat Mar 30, 2013 3:22 pm
 
Forum: Cell Biology
Topic: Western Blot
Replies: 6
Views: 3406

nuclear DNA binding

... DNA, if nothing is known about the binding preferences at all. Is there a standard that I might not be aware of? I thought that preparing nuclear extracts, performing IP against the protein (many antibodies are available) and checking DNA levels in the pull-downed material against negative (matrix ...

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by Balls
Tue Jan 22, 2013 9:03 am
 
Forum: Molecular Biology
Topic: nuclear DNA binding
Replies: 1
Views: 1932

Help Me To Solve These Question In general biology

... have a same ancestor. 4. Organic food is healthier than the other types of food. B-Questions:Answer them in scientific way: 1- How effective are extracts of marigold and rosemary as mosquitoes repellents?

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by luluwa999
Tue Sep 25, 2012 4:32 am
 
Forum: General Discussion
Topic: Help Me To Solve These Question In general biology
Replies: 0
Views: 1273

Idea to determine order of assembly of spliceosome

... 3. Extraction of pre-mRNA 4. Run agarose-gel where I can identify and roughly determine the size of my marked RNA 5. Add RNA to mammalian cell extracts, e.g. HeLa 6. While the RNA undergoes maturing, I collect samples with regular intervals of time 7. Use phenol to extract proteins from each ...

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by nukeman
Mon May 28, 2012 7:48 pm
 
Forum: Molecular Biology
Topic: Idea to determine order of assembly of spliceosome
Replies: 15
Views: 7821
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