Extraction

Hi there, I have to do a cell wall extraction on bacteria soon and my protein of interest is cell wall anchored. My supervisor told me to do TCA precipitation (haven't done before) but I assume it's too isolate the proteins within the cell ...

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... doing some reading, I have come up with a different plan for quantifying HIF-1 alpha, which involves running an ELISA after performing a nuclear extraction. However, all the protocols and kits I have seen recommend 1-10 million cells for a good extraction. I am likely going to be using much fewer ...

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How can you isolate any protein with Western blot? :roll: Trizol should work for protein extraction, but I have never used it. I guess they will be inactive if is it OK for you.

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... cells are not grown as 2D monolayers (I am looking at cell aggregates to be specific). Our original plan is to isolate the protein using trizol extraction reagent, then run an ELISA on HIF 1 alpha. However, I am worried that the protocol may not work. I haven't heard of trizol being used for ...

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Hello, I need to purify DNA from animals and use my own protocol instead of buying kits all the time. Can anybody suggest a good protocol to purify clean DNA which can be used in PCRs? Thanks.

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