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Extract

Extract

(Science: pharmacology) a concentrated preparation of a vegetable or animal drug obtained by removing the active constituents therefrom with a suitable menstruum, evaporating all or nearly all the solvent and adjusting the residual mass or powder to a prescribed standard. Extracts are prepared in three forms, semiliquid or of syrupy consistency, pilular or solid and as dry powder.

Origin: L. Extractum


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PCR & Agarose gel electrophoresis

... gel and the time of the run? Did you expose the RNA to light too much? It's possible you denatured them. Also, there may have been RNAses in your extract that could have reduced copy count drastically.

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by jyaron
Sat Sep 05, 2009 10:32 pm
 
Forum: Molecular Biology
Topic: PCR & Agarose gel electrophoresis
Replies: 3
Views: 120

Re: About getting a genetic sequence

... the organism vs. human beings. It's funny because I have an experiment as well with a totally different species but I don't know how to completely extract the DNA. But I do know about the Centrifuge with blood plasma Separation. I say this because the subject is more of a symbiotic species. I mean ...

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by CXCR4
Tue Aug 11, 2009 9:36 pm
 
Forum: Genetics
Topic: About getting a genetic sequence
Replies: 10
Views: 2463

Re: I get short smears trying to amplify tomato DNA, pleaseHelp

I have ordered samples of plant DNA extraction kits from both MoBio and Epicenter. I am looking forward to try those. I noticed that on the gel I ran for my genomic extract that there was much more intensity of bands that were very small (~50-400)bp ...

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by tomato
Tue Jul 28, 2009 7:41 pm
 
Forum: Molecular Biology
Topic: I get short smears trying to amplify tomato DNA, pleaseHelp
Replies: 5
Views: 165

RNA from feathers

Hello, Hope this post has a better outcome than my previous one which is still waiting for an answer! :P Do someone now how to extract RNA from feathers of adult birds? if so, can you suggest me some papers or methodologies to follow please? Many thanks in advance :) B

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by blurcita7
Tue Jul 21, 2009 3:33 pm
 
Forum: Molecular Biology
Topic: RNA from feathers
Replies: 3
Views: 138

Urgent:Algal Oil Extraction..suggestions

... am able to convert 50 mL of chlorella into 60L of the culture. But this is not the main purpose of this project. The main purpose is the algal oil extraction. Both these algae are said to have like 20-25% of lipids in it. I would be extracting that using soxhlet apparatus. But I am stuck at a very ...

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by vij1988
Fri Jul 17, 2009 2:28 am
 
Forum: Microbiology
Topic: Urgent:Algal Oil Extraction..suggestions
Replies: 1
Views: 183
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