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Extinction coefficient

Extinction coefficient

(Science: chemistry) a constant used in the beer-lambert law which relates the concentration of the substance being measured (in moles) to the absorbance of the substance in solution (how well the substance in solution blocks light beamed through it from getting out on the other side).


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Optical density Q

... prepared by dissolving 35mg of X in 50 ml of water. What is the molar extinction coefficient of X at 400nm? (mol. wt. of X is 450) (20% of the marks) (ii) Measurements of lactate ...

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by monkey07
Mon Jun 01, 2009 4:34 pm
 
Forum: Molecular Biology
Topic: Optical density Q
Replies: 0
Views: 409

Molar Extinction Coefficient Trouble!

nothing. ignore it. A=EbC where E is molar extinction coefficient, b is the path of light in cm, and C is concentration

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by alexandertg6
Wed Apr 22, 2009 12:31 pm
 
Forum: Molecular Biology
Topic: Molar Extinction Coefficient Trouble!
Replies: 1
Views: 290

Molar Extinction Coefficient Trouble!

... of BCG dye at 614 nm. I have been asked to estimate the molar extinction coefficient for BCG from the graph and show how my value was calculated. The Molecular weight ...

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by sophi3nutt
Tue Apr 21, 2009 11:38 am
 
Forum: Molecular Biology
Topic: Molar Extinction Coefficient Trouble!
Replies: 1
Views: 290

Re: Molar Extinction Coefficient

So if I have this data: Absorbance @ 405nm: .875 Concentration (M) of Cr(NO3)3 (aq): .25M Since you're saying that if l = 1cm (standard curvette), then E = A/C so then: .875/.25 = E E = 3.5?

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by r6barfly
Thu Mar 19, 2009 5:21 am
 
Forum: Molecular Biology
Topic: Molar Extinction Coefficient
Replies: 4
Views: 15776

Re: Beer Lambert Law

... what happened to your exponent on the power of ten? I didn't check the extinction coefficient (now preferably called the molar absorptivity) of NADH. I'll take your word for ...

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by blcr11
Thu Mar 05, 2009 2:42 pm
 
Forum: Molecular Biology
Topic: Beer Lambert Law
Replies: 1
Views: 701
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