Dictionary » E » Expression vectors

Expression vectors

(noun) Plural form of expression vector.

See expression vector for definition and additional information.


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Lac operon, e.coli expression vectors and biotechnology uses

How can gene regulation and expression be manipulated for designing expression vectors? And as a result, what are their potential biotechnological applications?

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by luna2000
Fri Jan 18, 2013 10:51 pm
 
Forum: Molecular Biology
Topic: Lac operon, e.coli expression vectors and biotechnology uses
Replies: 1
Views: 1061

overexpression xylanase gene

... me a project to conduct. can u help me understanding this project? overexpression of xylanase gene of bacillus (mutant and wild type) into e coli ... i perform this experiment? step by step if you may 2. how many type of vectors i have to use for this experiment? 3. how to check the activity of ...

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by nekonara
Thu Dec 13, 2012 6:59 am
 
Forum: Molecular Biology
Topic: overexpression xylanase gene
Replies: 2
Views: 1247

How to go about creating a stably-transfected cell line

... should be done. First off, how should I go about getting a suitable expression vector? I.e., what should I be looking for? I know that the vector should have a mammalian selection marker, and most mammalian expression vectors usually have a selection marker for bacterial systems too. What is ...

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by JustinNg
Mon Sep 17, 2012 9:26 am
 
Forum: Molecular Biology
Topic: How to go about creating a stably-transfected cell line
Replies: 1
Views: 1497

problem with expression vector cultivation

I use pfastBAC1 expression vector from invitrogen. I cultivate 15 mikroliter original vector to 200 ml ... the resistance gene. I wanted to stock the vector but ı have problems with these copy vectors. I do my ligations with this copy vectors that ı cultivated from original but none ...

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by genetherapy
Sat Mar 03, 2012 11:26 am
 
Forum: Molecular Biology
Topic: problem with expression vector cultivation
Replies: 1
Views: 1064

Regulation of gene expression in Bacteria

... the following: The lac promotor and repressor are commonly used in expression cloning vectors. But now my question is: Why? Is it because they are thouroughly studied and well know, ...

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by Lucanus cervus
Sat Jan 08, 2011 11:03 pm
 
Forum: Genetics
Topic: Regulation of gene expression in Bacteria
Replies: 2
Views: 1824
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