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Expression vector

Definition

noun, plural: expression vectors

A plasmid containing the required regulatory sequences specifically used for the expression of a particular gene into proteins within the target cell.


Supplement

The expression vector is a plasmid engineered to introduce a particular gene into the target cell. The plasmid contains the regulatory sequences that serve as enhancer and promoter regions needed for the expression of a specific gene using the transcription and translation machinery of the target cell.

An example of expression vector is the plasmid used to produce insulin important for treating diseases such as diabetes. Another example is the expression vector that introduces specific genes necessary for the synthesis of beta-carotene into the cells of rice plants, giving rise to a new variety called golden rice.


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Gateway technology

... the gateway technology is a lab method to obtain cloning and expression vectors based on the property of phages to recombine its genetic material with bacterias. However, ...

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by miibay
Tue Aug 19, 2014 10:47 pm
 
Forum: Molecular Biology
Topic: Gateway technology
Replies: 1
Views: 74

cDNA cloning and Lentiviral transfection

... in them. I have this mcherry labelled integrin cDNA in a mammalian expression vector. If my understanding is correct, I can transfect cells with this vector directly to ...

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by rkrishna
Thu Jun 27, 2013 11:22 pm
 
Forum: Molecular Biology
Topic: cDNA cloning and Lentiviral transfection
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Views: 825

Gene Cloning

Since a PCR product can insert in both direction in the PTZ57 vector and both of clones can grow in a ampicillin LB plates, How can we find the clone that has the correct direction for expression of PCR product?

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by ghaemi100
Fri Jun 21, 2013 9:39 am
 
Forum: Molecular Biology
Topic: Gene Cloning
Replies: 3
Views: 1355

Producing unknown protein

... protein sequence to DNA sequence. 2. Codon optimize to the expression system (you have a choice of bacterial or cell free expression systems) 3. Synthesize sequence (IDT can do it for you). 4. Ligate into vector (vector depends on the expression system)/clone vector. 3. Transform ...

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by Cat
Mon May 20, 2013 1:56 pm
 
Forum: Microbiology
Topic: Producing unknown protein
Replies: 2
Views: 2016

Orientation of genes

... needed. Sorry When making a gene construct (before inserting into an expression vector), do we have to place the desired gene, the reporter gene and the marker gene in any ...

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by Sue4
Mon Jan 28, 2013 9:03 pm
 
Forum: Molecular Biology
Topic: Orientation of genes
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Views: 1144
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