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Expression system

Expression system

(Science: molecular biology) combination of an expression vector, its cloned dna, and the host for the vector that provide a context to allow foreign gene function in a host cell, that is, produce proteins at a high level.


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Re: Really puzzled with gene expresseion!!!!

The bacterial system controlling expression of the genes needed to take up and use lactose is a classic example of the regulation of genes in ...

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by jonmoulton
Mon Apr 27, 2009 3:07 pm
 
Forum: Genetics
Topic: Really puzzled with gene expresseion!!!!
Replies: 4
Views: 224

question about shRNA knocking down

... of the potential problems. You could express your shRNA in a cell-free system then transfect the cells. This at least would eliminate some of the ... and you can determine whether the shRNA sequence is failing or its expression in the cells is the source of the problem.

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by jonmoulton
Mon Dec 01, 2008 4:57 pm
 
Forum: Molecular Biology
Topic: question about shRNA knocking down
Replies: 1
Views: 394

Re: Developmental Biology-I need help

... Katow H. Spatio-temporal expression of a Netrin homolog in the sea urchin Hemicentrotus pulcherrimus ... mediates guidance events controlling morphogenesis of the vascular system. Nature. 2004 Nov 11;432(7014):179-86. Epub 2004 Oct 27. Wang GX, ...

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by jonmoulton
Fri Nov 07, 2008 7:03 pm
 
Forum: Molecular Biology
Topic: Developmental Biology-I need help
Replies: 1
Views: 419

Re: is it possible to knockdown a gene in a living adult now

... Cell-penetrating peptide-conjugated antisense oligonucleotides restore systemic muscle and cardiac dystrophin expression and function. Hum Mol Genet. 2008 Sep 10. [Epub ahead of print] Wu B, Moulton HM, ...

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by jonmoulton
Fri Nov 07, 2008 3:44 pm
 
Forum: Genetics
Topic: is it possible to knockdown a gene in a living adult now
Replies: 10
Views: 1164

Re: Allele symbols/acronyms?

Sorry, wrong system. For some reason—and now that I look again at the original question, I don’t know ... under non-permissive conditions you may see the effect of the absence of the gene’s expression. For the fl/fl cells, in the absence of Cre recombinase, Rb is present, just ...

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by blcr11
Wed Sep 10, 2008 1:25 am
 
Forum: Genetics
Topic: Allele symbols/acronyms?
Replies: 7
Views: 1232
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