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Expression system

Expression system

(Science: molecular biology) combination of an expression vector, its cloned dna, and the host for the vector that provide a context to allow foreign gene function in a host cell, that is, produce proteins at a high level.


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mRNA vs. cell reporter assay

... same question, phrased in a slightly different way: Maybe the reporter system provides us with more information than RT-PCR/Western blot, because ... present? I thought it is enough to detect a significant increase in the expression by Western blot and we could conclude that the particular transcription ...

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by kk
Mon Sep 08, 2014 2:57 pm
 
Forum: Cell Biology
Topic: mRNA vs. cell reporter assay
Replies: 2
Views: 383

Re: mammary gland of male in mammals

... in prolactin. The lobes of the mammalian breast are part of the duct system which pushes the milk through to the nipple; the alveoli produce colostrum ... necessary anti bodies for the maturing infant. So, when you hear the expression, the size of the breast doesn't matter, a woman can still successfully ...

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by Annij
Sun Nov 03, 2013 4:47 am
 
Forum: Evolution
Topic: mammary gland of male in mammals
Replies: 18
Views: 17800

Locus specificity and ERVs - Q from a creationist

... an adaptive function. It seems like the adaptive function is non-expression, which might be affected by location, or might not. If the ERVs are in similar locations, that would imply that they entered the system in a shared ancestor...

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by Darby
Tue Sep 10, 2013 3:57 pm
 
Forum: Genetics
Topic: Locus specificity and ERVs - Q from a creationist
Replies: 5
Views: 2097

Producing unknown protein

... protein sequence to DNA sequence. 2. Codon optimize to the expression system (you have a choice of bacterial or cell free expression systems) 3. Synthesize sequence ...

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by Cat
Mon May 20, 2013 1:56 pm
 
Forum: Microbiology
Topic: Producing unknown protein
Replies: 2
Views: 2053

Bio Research Paper Writing

... senescence relate to aging as a whole? Abstract Age related immune-system decline in function may be more than self inflicted damage caused ... defense such as cell surface markers. CMV and adenoviruses decrease the expression of a cell’s MHC recognition markers which decreases the ability ...

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by NathanielZhu
Thu Apr 04, 2013 2:25 am
 
Forum: General Discussion
Topic: Bio Research Paper Writing
Replies: 1
Views: 12239
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