Expression system

The bacterial system controlling expression of the genes needed to take up and use lactose is a classic example of the regulation of genes in ...

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... of the potential problems. You could express your shRNA in a cell-free system then transfect the cells. This at least would eliminate some of the ... and you can determine whether the shRNA sequence is failing or its expression in the cells is the source of the problem.

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... Katow H. Spatio-temporal expression of a Netrin homolog in the sea urchin Hemicentrotus pulcherrimus ... mediates guidance events controlling morphogenesis of the vascular system. Nature. 2004 Nov 11;432(7014):179-86. Epub 2004 Oct 27. Wang GX, ...

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... Cell-penetrating peptide-conjugated antisense oligonucleotides restore systemic muscle and cardiac dystrophin expression and function. Hum Mol Genet. 2008 Sep 10. [Epub ahead of print] Wu B, Moulton HM, ...

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Sorry, wrong system. For some reason—and now that I look again at the original question, I don’t know ... under non-permissive conditions you may see the effect of the absence of the gene’s expression. For the fl/fl cells, in the absence of Cre recombinase, Rb is present, just ...

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