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Expression system

Expression system

(Science: molecular biology) combination of an expression vector, its cloned dna, and the host for the vector that provide a context to allow foreign gene function in a host cell, that is, produce proteins at a high level.


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UWM is seeking testers for a new protein expression kit

... that has traditionally been studied for its simple photosynthetic system, has demonstrated recent success in expressing high-yield protein. ... normal cellular function. This characteristic has stimulated the expression of active and correctly folded membrane protein where other systems ...

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by ejhuwmfdn
Thu Feb 14, 2013 3:05 pm
 
Forum: Molecular Biology
Topic: UWM is seeking testers for a new protein expression kit
Replies: 0
Views: 270

A question I'm finding hard to answer (recombinant protein)

... do this for the protein in the original species are also present in the expression system, even if the expression system is another eukaryote. For prokaryotes (bacteria), this ...

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by AstraSequi
Mon Apr 30, 2012 5:39 pm
 
Forum: Cell Biology
Topic: A question I'm finding hard to answer (recombinant protein)
Replies: 9
Views: 3383

using uniprot

... lysozyme-like genes, predominantly expressed in the male reproductive system of humans, belonging to the c-type lysozyme/alpha-lactalbumin family"(reference), ... " P61626 " is from " Cloning of human lysozyme gene and expression in the yeast Saccharomyces cerevisiae." ...

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by jmagicking
Fri Apr 06, 2012 7:36 am
 
Forum: Bioinformatics
Topic: using uniprot
Replies: 3
Views: 1773

Theories - Origin of Life

... nobody has yet gone through all of them (a difficult undertaking) to systematically record their similarities and differences and propose a new ... in the nucleus and thereby determines the way and timing of how expression takes place. We also know chromatin structure, the variation in ...

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by AstraSequi
Tue Mar 27, 2012 2:41 am
 
Forum: Evolution
Topic: Theories - Origin of Life
Replies: 548
Views: 534366

SOS RESPONSE! Recombinant DNA

... is because of the differences between the eukaryotic and bacterial systems, which I think you understand (so, for example, you would want to ... out of the DNA before inserting it in your vector). In a eukaryotic expression system, then these problems will not be as bad because you will ...

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by AstraSequi
Sun Feb 12, 2012 1:44 am
 
Forum: Genetics
Topic: SOS RESPONSE! Recombinant DNA
Replies: 1
Views: 733
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