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Expression site

Expression site

The location in the genome of the gene for the variable surface glycoprotein that is currently being expressed (an expression-linked copy) by the trypanosome (a parasitic protozoan which causes the disease african sleeping sickness). most of these sites are near the ends, or telomeres, of a chromosome.


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Re: Any ideas for vaccine development or anti-viral drugs???

... A 22-mer P-PMO designed to base pair with the translation start site region of EBOV VP35 positive-sense RNA generated sequence-specific and ... activity reduced budding by up to 80%. Inhibition of VPS4 gene expression by use of phosphorodiamidite morpholino antisense oligonucleotides ...

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by jonmoulton
Mon Sep 15, 2014 3:04 pm
 
Forum: Cell Biology
Topic: Any ideas for vaccine development or anti-viral drugs???
Replies: 2
Views: 504

Gene knockdowns in the wasp Nasonia vitripennis

... by microinjection. Dual mode of embryonic development is highlighted by expression and function of Nasonia pair-rule genes. Rosenberg MI, Brent AE, ... we designed an Nv eve morpholino overlapping the translation start site, as well as one directed at the exon-intron junction in the homeobox. ...

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by jonmoulton
Fri Mar 07, 2014 5:57 pm
 
Forum: Molecular Biology
Topic: Gene knockdowns in the wasp Nasonia vitripennis
Replies: 0
Views: 1167

Real Time reverse transcription PCR Questions

... is the same orientation as DNA right? For example, lets say the NCBI website gives me the following sequence (please disregard if they are good primers ... method for Real Time PCR, I am using this method to detect the expression levels (upregulated or downregulated) of mRNA in different samples, ...

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by wessH
Sun Jun 03, 2012 2:21 pm
 
Forum: Molecular Biology
Topic: Real Time reverse transcription PCR Questions
Replies: 0
Views: 2490

Transcription Factor Question

... are controled by this TF (without being interested in actual binding site), you need to change it's expression and see, what changes in transcription of other genes you get ;)

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by JackBean
Wed Apr 18, 2012 6:32 am
 
Forum: Molecular Biology
Topic: Transcription Factor Question
Replies: 7
Views: 2582

How to replace gene with modified gene [unequal length] ?

... longer than the original gene ? I know one can put the sequence into an expression vector or some type of virus but that would be introducing the gene into a diffeent site while the original gene is still around . Is there any mechanism that would allow ...

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by Hicsuntdrac0nis
Mon Mar 19, 2012 5:38 am
 
Forum: Genetics
Topic: How to replace gene with modified gene [unequal length] ?
Replies: 3
Views: 1923
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