Expression site

... is the same orientation as DNA right? For example, lets say the NCBI website gives me the following sequence (please disregard if they are good primers ... method for Real Time PCR, I am using this method to detect the expression levels (upregulated or downregulated) of mRNA in different samples, ...

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... are controled by this TF (without being interested in actual binding site), you need to change it's expression and see, what changes in transcription of other genes you get ;)

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... longer than the original gene ? I know one can put the sequence into an expression vector or some type of virus but that would be introducing the gene into a diffeent site while the original gene is still around . Is there any mechanism that would allow ...

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... (maybe it's just a reprint on ID site, but that's where the Scottie's opinion in this case came from). I’ve ... thinking by the “majority” argument anyway? Even in science there is an expression “Science advances one funeral at a time”. And outside of science, ...

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... but now I have to design two outer primers to clone the gene into a pET expression vector and I have no idea how! I've pretty much been staring at ... I might have just had a breakthrough thanks to trusty wikipedia, on the site directed mutagenesis wiki page it says "Variations employ three ...

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