Expression construct

"to be cloned" part is not needed. Sorry When making a gene construct (before inserting into an expression vector), do we have to place the desired gene, the reporter gene and the marker ...

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I know that a reporter gene can be used to detect the foreign gene expression if it is fused with the foreign gene. But can we monitor the foreign ... use of placing a selectable marker and a reporter gene under the same construct (without a fusion) ? Thank you Regards :)

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... have restriction sites of interest, digest/insert into intermediate/expression construct, purify DNA from selected clones, check, sequence and produce proteins of importance. ...

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Why do you use the promoter region? If you looking gene expression you need to do it on cDNA using Q-PCR if you want to make a reporter construct then you want to PCR out the promoter region and put it in front of a reporter gene such ...

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... Neomycin gene products and 293 cells do not. Otherwise, overall protein expression the same. We transfect the cells with a cDNA construct. We perform transfection based on the cDNA collection that we are working with. So ...

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