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Expression cloning

Expression cloning

(Science: molecular biology) a vector that results in the expression of inserted dna sequences when propagated in a suitable host cell, i.e. The protein coded for by the dna is synthesised by the hosts system.


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cDNA cloning and Lentiviral transfection

... in them. I have this mcherry labelled integrin cDNA in a mammalian expression vector. If my understanding is correct, I can transfect cells ... with cDNA, and obtain copies of it? As in, can I use this vector for cloning in bacteria? Else, where do I start? Also, I am considering lentiviral ...

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by rkrishna
Thu Jun 27, 2013 11:22 pm
 
Forum: Molecular Biology
Topic: cDNA cloning and Lentiviral transfection
Replies: 1
Views: 574

Gene Cloning

... direction in the PTZ57 vector and both of clones can grow in a ampicillin LB plates, How can we find the clone that has the correct direction for expression of PCR product?

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by ghaemi100
Fri Jun 21, 2013 9:39 am
 
Forum: Molecular Biology
Topic: Gene Cloning
Replies: 3
Views: 1068

Re:

... and look at the splicing nature of ligase 6 before we re-attempt any cloning or over-expression. In order to investigate the splicing nature of the gene, I created primers for ...

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by polkadotshorts
Mon Dec 10, 2012 7:12 pm
 
Forum: Genetics
Topic: How to clone a gene with lots of splicing intermediates?
Replies: 6
Views: 2556

Integrated software tools

... Sequencing view Primer design in silico PCR Mutagenesis Virtual cloning Plasmid maps UGENE can do most of these things but its focus on 'projects' ... have restriction sites of interest, digest/insert into intermediate/expression construct, purify DNA from selected clones, check, sequence and ...

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by dustman
Tue May 08, 2012 12:17 pm
 
Forum: Molecular Biology
Topic: Integrated software tools
Replies: 4
Views: 1794

A question I'm finding hard to answer (recombinant protein)

... Can you add anymore to the answer (improve it) ? A recombinant protein expression experiment involves cloning foreign DNA into a vector for expression of the protein (encoded by the cloned gene) ...

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by ibz52002
Sat Apr 28, 2012 4:45 pm
 
Forum: Cell Biology
Topic: A question I'm finding hard to answer (recombinant protein)
Replies: 9
Views: 5792
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