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Expression cloning

Expression cloning

(Science: molecular biology) a vector that results in the expression of inserted dna sequences when propagated in a suitable host cell, i.e. The protein coded for by the dna is synthesised by the hosts system.


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An Overview of Embryonic Stem Cells

... out’ and ‘knock in’ transgenic animals for the investigation of gene expression and regulation in vivo. Many efforts have also been made to isolate ... for deriving human ES cells, namely, the somatic nuclear transfer, or cloning, which bypasses the need to destroy an embryo produced by the fusion ...

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by 2444256703
Thu Jan 29, 2015 6:48 am
 
Forum: Cell Biology
Topic: An Overview of Embryonic Stem Cells
Replies: 1
Views: 574

Gateway technology

From what I understand, the gateway technology is a lab method to obtain cloning and expression vectors based on the property of phages to recombine its genetic material with bacterias. ...

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by miibay
Tue Aug 19, 2014 10:47 pm
 
Forum: Molecular Biology
Topic: Gateway technology
Replies: 1
Views: 1772

cDNA cloning and Lentiviral transfection

... in them. I have this mcherry labelled integrin cDNA in a mammalian expression vector. If my understanding is correct, I can transfect cells ... with cDNA, and obtain copies of it? As in, can I use this vector for cloning in bacteria? Else, where do I start? Also, I am considering lentiviral ...

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by rkrishna
Thu Jun 27, 2013 11:22 pm
 
Forum: Molecular Biology
Topic: cDNA cloning and Lentiviral transfection
Replies: 1
Views: 1378

Gene Cloning

... direction in the PTZ57 vector and both of clones can grow in a ampicillin LB plates, How can we find the clone that has the correct direction for expression of PCR product?

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by ghaemi100
Fri Jun 21, 2013 9:39 am
 
Forum: Molecular Biology
Topic: Gene Cloning
Replies: 3
Views: 1894

Re:

... and look at the splicing nature of ligase 6 before we re-attempt any cloning or over-expression. In order to investigate the splicing nature of the gene, I created primers for ...

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by polkadotshorts
Mon Dec 10, 2012 7:12 pm
 
Forum: Genetics
Topic: How to clone a gene with lots of splicing intermediates?
Replies: 6
Views: 4444
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