Expression-linked copy

"Does this imply that all the somatic cells in the mice contain 1 copy of the functional gene and 1 copy of the non-functional gene? " ... is on the X sex chromosome (where chromosome silencing can complicate expression). "What confuses me is that if this is the case, then what's ...

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... gene. The mouse will likely produce the protein from the wild-type copy of the gene, but depending on how the gene is regulated it is likely that expression of the protein will be below wild-type levels.

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... together, so you do cross-pollination. Your new generation receives one copy of each chromosome from yellow pea plant and one copy of each chromosome ... subtle way or drastically on the DNA level – the main difference is in expression. That can be due to different transcription efficiencies (mRNA), ...

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... routine is to pick a gene, already pre-cloned in some sort of plasmid, copy a fragment with PCR, where primers have restriction sites of interest, digest/insert into intermediate/expression construct, purify DNA from selected clones, check, sequence and produce ...

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I use pfastBAC1 expression vector from invitrogen. I cultivate 15 mikroliter original vector to 200 ml ... have the resistance gene. I wanted to stock the vector but ı have problems with these copy vectors. I do my ligations with this copy vectors that ı cultivated from original ...

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