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Expression

Expression

(Science: molecular biology) The process by which a genes coded information is converted into the structures present and operating in the cell.

expressed genes include those that are transcribed into mrna and then translated into protein and those that are transcribed into rna but not translated into protein (for example, transfer and ribosomal RNAs).


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mRNA vs. cell reporter assay

... it proves that the transcription factor is actually active too, not just present? I thought it is enough to detect a significant increase in the expression by Western blot and we could conclude that the particular transcription factor is more active... do TFs need activity assays to be validated?

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by kk
Mon Sep 08, 2014 2:57 pm
 
Forum: Cell Biology
Topic: mRNA vs. cell reporter assay
Replies: 2
Views: 381

Gateway technology

From what I understand, the gateway technology is a lab method to obtain cloning and expression vectors based on the property of phages to recombine its genetic material with bacterias. However, why do I need to perform the BP reaction, if I want to obtain an expression ...

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by miibay
Tue Aug 19, 2014 10:47 pm
 
Forum: Molecular Biology
Topic: Gateway technology
Replies: 1
Views: 407

Re: Totipotent Stem Cell From Molecular Components

... case). Rather I am saying that for a particular protein in my stem cell at least, the amount of that particular protein is the average of the gene expression in # of strands of mRNA * 100. I am getting the protein this way. I take the results of PCR and put it in a test tube with RNA polymerases, ...

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by caters
Fri Aug 01, 2014 1:42 am
 
Forum: Cell Biology
Topic: Totipotent Stem Cell From Molecular Components
Replies: 2
Views: 2760

Trying to prove height increase...need help

... much more load than is present in the study(fairly intense clamping). Possible reasons could be related to hydrostatic pressure, changes in gene expression in stem cells resulting in differentiation, osteoclast degradation enabling interstitial growth, or gene signaling from the articular cartilage/synovial ...

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by MiniGolfer
Thu Jul 24, 2014 9:09 pm
 
Forum: General Discussion
Topic: Trying to prove height increase...need help
Replies: 2
Views: 559

mRNA vs. cell reporter assay

Hi, I have a GFP reporter cell line that drives GFP expression upon activation of a transcription factor. In theory, I can study transcription factor activation via the GFP signal, or by measuring the mRNA of the transcription factor. I wonder what ...

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by aguy
Mon Jul 07, 2014 2:50 am
 
Forum: Cell Biology
Topic: mRNA vs. cell reporter assay
Replies: 2
Views: 381
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