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Expression

Expression

(Science: molecular biology) The process by which a genes coded information is converted into the structures present and operating in the cell.

expressed genes include those that are transcribed into mrna and then translated into protein and those that are transcribed into rna but not translated into protein (for example, transfer and ribosomal RNAs).


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Gene Upregulation/Increased Expression in HMG-CoA Reductase

... However, in a class the other day a professor tried to differentiate the two and left me pretty confused. From my understanding, increased gene expression will result in an increase in the protein product that a gene is coding for. Meaning that if you increased expression of a gene that codes ...

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by Upperninety8
Tue Sep 30, 2014 3:21 am
 
Forum: Genetics
Topic: Gene Upregulation/Increased Expression in HMG-CoA Reductase
Replies: 0
Views: 47

Re: translation from DNA

... steps in the process; we must infer the process from observing modern biochemistry. However, I can speculate that much of the regulation of gene expression occurs at the RNA level, with variations in the stability of RNA, translational repression by mRNA, inhibition by natural antisense transcripts, ...

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by jonmoulton
Mon Sep 29, 2014 3:03 pm
 
Forum: Molecular Biology
Topic: translation from DNA
Replies: 2
Views: 224

Re: Any ideas for vaccine development or anti-viral drugs???

... independently of TSG101 interaction. A lack of VPS4 adenosine triphosphatase activity reduced budding by up to 80%. Inhibition of VPS4 gene expression by use of phosphorodiamidite morpholino antisense oligonucleotides protected mice from lethal EBOV infection. These data show that EBOV can ...

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by jonmoulton
Mon Sep 15, 2014 3:04 pm
 
Forum: Cell Biology
Topic: Any ideas for vaccine development or anti-viral drugs???
Replies: 2
Views: 478

mRNA vs. cell reporter assay

... it proves that the transcription factor is actually active too, not just present? I thought it is enough to detect a significant increase in the expression by Western blot and we could conclude that the particular transcription factor is more active... do TFs need activity assays to be validated?

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by kk
Mon Sep 08, 2014 2:57 pm
 
Forum: Cell Biology
Topic: mRNA vs. cell reporter assay
Replies: 2
Views: 693

Gateway technology

From what I understand, the gateway technology is a lab method to obtain cloning and expression vectors based on the property of phages to recombine its genetic material with bacterias. However, why do I need to perform the BP reaction, if I want to obtain an expression ...

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by miibay
Tue Aug 19, 2014 10:47 pm
 
Forum: Molecular Biology
Topic: Gateway technology
Replies: 1
Views: 604
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