Dictionary » E » Experiments



Noun: a procedure done in a controlled environment for the purpose of gathering observations, data, or facts, demonstrating known facts or theories, or testing hypotheses or theories. Verb: to carry out such a procedure.

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Re: how to cultrue IEC-6

... says: DMEM + 2mM Glutamine + 0.1 IU/ml Insulin + 5% Foetal Bovine Serum (FBS); 5% CO2 Different people use different protocols for different experiments. Sometimes they grow fine in either, but change slightly which makes one version more suitable for your experiments. If you don't know, ...

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by Tricho
Thu Mar 26, 2015 10:08 pm
Forum: Cell Biology
Topic: how to cultrue IEC-6
Replies: 1
Views: 15

gene knockout mice

... and a fluorescent marker and inject stereotactically in the striatum. This will not be 100% efficient, but might be sufficient for certain experiments. With neurons it is easier to restrict to specific brain regions, they tend to be more locally different and specific than glial cells. ...

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by animodel123
Wed Mar 18, 2015 6:46 am
Forum: Genetics
Topic: gene knockout mice
Replies: 1
Views: 238

Methylene Blue Solution use in staining variety of bacteria?

... pathogenic. Gram negative bacterial cell walls are composed of lipopolysaccharides that can be an endotoxin (toxic shock syndrome). Gram stain experiments are another type of a multi-step staining and de-staining experiment. Reagents 0.1M Sodium Acetate Buffer, pH4.2: Sodium acetate, trihydrate ...

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by scytek
Sun Feb 22, 2015 9:22 am
Forum: General Discussion
Topic: Methylene Blue Solution use in staining variety of bacteria?
Replies: 0
Views: 271

Beginner Microbiologist, looking for a place to start.

... I have done all the basics- looked at pond water, plants, I have grown bacteria in Agar plates. I am at a point where I want to start doing experiments or research that contribute to science in some way. Does anyone have any ideas? I just need something to do to keep me interested in microbiology. ...

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by fstevens
Sat Dec 13, 2014 3:30 pm
Forum: General Discussion
Topic: Beginner Microbiologist, looking for a place to start.
Replies: 1
Views: 1079

TTEST help!

... a knockdown cell pool using shRNA against my target gene and control. i then subject these cells to the same conditions (DNA damage, etc). I run experiments in triplicate usually, and then generate them independently 3 times. i have some really basic questions, if you could help that would be ...

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by hopeless123
Fri Nov 07, 2014 6:08 pm
Forum: General Discussion
Topic: TTEST help!
Replies: 0
Views: 628
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