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Experiment

Experiment

Noun: a procedure done in a controlled environment for the purpose of gathering observations, data, or facts, demonstrating known facts or theories, or testing hypotheses or theories. Verb: to carry out such a procedure.


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A question about what living organisms are made up of.

Wikipedia is your friend http://en.wikipedia.org/wiki/Endosymbiotic_theory#History http://en.wikipedia.org/wiki/Primordial_soup http://en.wikipedia.org/wiki/Miller%E2%80%93Urey_experiment http://en.wikipedia.org/wiki/Abiogenesis#Current_models

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by JackBean
Mon Mar 10, 2014 3:37 pm
 
Forum: Molecular Biology
Topic: A question about what living organisms are made up of.
Replies: 8
Views: 461

Mendel's law

If the experiment wasn't controlled properly the plants could cross fertilise so you might not be observing a pure F1 generation e.g. an F1 could cross with the parent plant which could change the phenotypic ratios viewed in ...

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by laurak120
Thu Mar 06, 2014 3:47 pm
 
Forum: Genetics
Topic: Mendel's law
Replies: 2
Views: 1330

Re: Western transfer failure

... lack of SDS mess with electric gradient? Correlation may not be causation, so I don't claim lack of SDS was the problem. It would be easy to do an experiment, though.

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by yanggers
Thu Feb 06, 2014 9:16 pm
 
Forum: Molecular Biology
Topic: Western transfer failure
Replies: 5
Views: 3992

Re: blank and substrate control give OD reads

... well plate 9) add 100ul DNS solution, incubate @ 100oC, 5min 10) pipet 100ul and transfer into multiwell plate to read @ OD540 >>> each experiment is done with blank (buffer sodium acetate only), substrate control (substrate + buffer) and wild type xylanase (with substrate and buffer) ...

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by intandiana
Sun Jan 19, 2014 5:57 am
 
Forum: Molecular Biology
Topic: blank and substrate control give OD reads
Replies: 9
Views: 1159

blank and substrate control give OD reads

... reaction is centrifuged to collect only reaction solution (without the substrate) then stop solution with DNS and incubate at 100oC, 5min. each experiment is controlled by blank (buffer only), substrate (buffer + substrate) and wild type xylanase. the problem is the blank and substrate control ...

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by intandiana
Wed Jan 15, 2014 7:21 am
 
Forum: Molecular Biology
Topic: blank and substrate control give OD reads
Replies: 9
Views: 1159
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