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Exons

Exons

coding regions of messenger rna included in the genetic transcript which survive the processing of rna in cell nuclei to become part of a spliced messenger of structural rna in the cytoplasm. They include joining and diversity exons of immunoglobulin genes.


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Re: An interesting PCR smearing...I dont know what to do :(

hello all , Even i have a similar problem. I am using 3 different primers to amplify 3 different exons of a gene . 1 of the exon is amplifying very well ( a single bright band ) but the other two exon amplification is not coming properly.and this has occured recently. ( ...

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by priyah
Sat Jun 28, 2014 5:20 pm
 
Forum: Molecular Biology
Topic: An interesting PCR smearing...I dont know what to do :(
Replies: 9
Views: 6529

Re: qPCR vs Northern Blot

... first - in Northern blot I had silencing, in qPCR i didn't. As far as your question is concerned - my NB probe covers whole length of mRNA - all exons (it's a short one ~2 kbp total). In case of my PCR primers - FORWARD primer binds in one exon in the middle of mRNA, and REVERSE binds at exon-exon ...

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by Nevthonien
Tue Nov 12, 2013 2:47 pm
 
Forum: Molecular Biology
Topic: qPCR vs Northern Blot
Replies: 4
Views: 2578

qPCR vs Northern Blot

Are your primers designed to bind on two different exons? @jonmoulton: if it was just partially degraded, there would be change in size of the bound RNA

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by JackBean
Mon Nov 11, 2013 1:28 pm
 
Forum: Molecular Biology
Topic: qPCR vs Northern Blot
Replies: 4
Views: 2578

Cell and Molecular Revision Question Help!!! (2)

... as to how to do them so I may learn. ESTIMATING GENE NUMBERS Approximately 1% of the human genome consists of protein-coding sequences (exons). A diploid somatic human cell contains 5 picograms/pg of DNA and rapidly growing E.coli cells contain on average four genomes giving a total ...

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by Didact1
Mon Sep 30, 2013 11:35 am
 
Forum: Cell Biology
Topic: Cell and Molecular Revision Question Help!!! (2)
Replies: 0
Views: 1923

Cell and Molecular Revision Question Help!!!

... as to how to do them so I may learn. ESTIMATING GENE NUMBERS Approximately 1% of the human genome consists of protein-coding sequences (exons). A diploid somatic human cell contains 5 picograms/pg of DNA and rapidly growing E.coli cells contain on average four genomes giving a total ...

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by Didact1
Mon Sep 30, 2013 11:34 am
 
Forum: Molecular Biology
Topic: Cell and Molecular Revision Question Help!!!
Replies: 4
Views: 2667
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