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Excitation

Excitation

(Science: physics, psychology) An act of irritation or stimulation or of responding to a stimulus, the addition of energy, as the excitation of a molecule by absorption of photons.

Origin: L. Excitatio, citare = to call


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Depolarization = ion channel opening?

... a problem with this statement: "Action potentials are formed when a stimulus causes the cell membrane to depolarize past the threshold of excitation, causing all sodium ion channels to open." Isn't the cell membrane depolarized BECAUSE the ion channels open?

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by autumnsphere
Wed Oct 29, 2014 2:18 pm
 
Forum: Cell Biology
Topic: Depolarization = ion channel opening?
Replies: 1
Views: 660

hybridization probe

I'd say it depends solely on your possibilities with the machine (excitation and detection range), ootherwise it shouldn't matter much.

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by JackBean
Tue Sep 17, 2013 3:43 pm
 
Forum: Genetics
Topic: hybridization probe
Replies: 2
Views: 2091

Re: Resonance transfer

... In FRET, a fluorescent molecule (fluorochrome) is excited with light; for now, let's say it's blue light. Instead of simply emitting the excitation energy at a longer wavelength, let's say green light, it instead passes the energy (the "exciton") to another fluorochrome that ...

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by jonmoulton
Fri Jun 07, 2013 3:20 pm
 
Forum: General Discussion
Topic: Resonance transfer
Replies: 1
Views: 2052

Re: Device for ratiometric fluorescence measurement

... the spectrum or rapidly changing ex/em bands, you would build a 2-band fluorescence colorimeter. You'd need two emission filters and one or two excitation filters, arranged around your cuvette so that the excitation and emission light paths intersect at a 90 degree angle in the sample. Of course, ...

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by jonmoulton
Mon Mar 04, 2013 4:58 pm
 
Forum: General Discussion
Topic: Device for ratiometric fluorescence measurement
Replies: 1
Views: 1808

Re: Tools in Biology

... detectable concentrations of fluorescent material in a sample and how the material is distributed in the sample. However, to check for different excitation wavelengths and emission colors, you must switch part of the optics in the microscope. This is easy in most fluorescence microscope as you ...

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by jonmoulton
Wed Mar 21, 2012 8:52 pm
 
Forum: General Discussion
Topic: Microscopy
Replies: 2
Views: 2375
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