Epitope

... is influenced by the peptide sequence surrounding the 6xHis tag. I know that these antibodies are usually successful at recognizing an MHHHHHH.... epitope, but I don't know if they would recognize MWRKRKRHHHHH, for example. I would contact the antibody supplier because it seems like this might ...

See entire post

... the blood and be consumed by a macrophage; if the pathogen is a virus it will enter into a cell and eventually be presented on that cell as an epitope+MHCI protein. Otherwise the macrophage will then degrade the pathogen into epitopes and present them on its surface bound to MHCII proteins. ...

See entire post

Signal amplification : more than one secondary antibody can detect the first one, so you have one fluororfore per epitope.

See entire post

... J 9:234-240 Schenkman S, Jiang MS, Hart GW, Nussensweig V (1991) A novel cell surface trans-sialidase of T. cruzi generates a stage-specific epitope required for invasion of mammalian cells. Cell 65:1117-1125 Silvia ZM, Julio CV, Carlos CM, Arturo FC, Everardo LR (1999) Entamoaeba histolytica: ...

See entire post

... Pat. Nos. 5,225,539; 5,530,101; and 5,585,089), veneering or resurfacing?????????????????? Completely human antibodies which recognize a selected epitope can be generated using a technique referred to as "guided selection." In this approach a selected non-human monoclonal antibody. The ...

See entire post