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Epitope

Epitope

(Science: immunology) That part of an antigenic molecule to which the t-cell receptor responds, a site on a large molecule against which an antibody will be produced and to which it will bind.

See: agretope.


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Re: In Vitro Transcription Translation

... unambiguously prove the identity of the protein you might need a Western as well, since your radiolabelled gel gives mass information but not an epitope-antigen interaction. If you have a single mRNA produced in the transcription-translation system then that simplifies the system. Without knowing ...

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by jonmoulton
Wed Jun 05, 2013 10:59 pm
 
Forum: Molecular Biology
Topic: In Vitro Transcription Translation
Replies: 3
Views: 1976

Creation of virus?

... talking about detection in enviroment or in human after infection/death? I cannot imagine, why it couldn't be designed that way. If you know the epitope, you can simply delete/alter it in such way, it will be recognized by different antibodies

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by JackBean
Sun Mar 27, 2011 11:31 am
 
Forum: Microbiology
Topic: Creation of virus?
Replies: 10
Views: 4525

simple epitope

I learned that there is a lower limit to the size of any potential epitope - is that what this is about-?

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by Darby
Tue Jan 18, 2011 8:50 pm
 
Forum: General Discussion
Topic: simple epitope
Replies: 5
Views: 1970

Re: simple epitope

... for your reply. this is not what the thing is about. I need something like a positive control - a known antibody known to bind a very simple epitope (best would be a loop - hence a nonlinear peptide or protein-fragment) which can be used in in-vitro experiments. Cheers, Chris

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by derChronostat
Tue Jan 18, 2011 11:16 am
 
Forum: General Discussion
Topic: simple epitope
Replies: 5
Views: 1970

simple epitope

you should be able to to raise antibodies only against your epitope (understand - use only that epitope for imunization), or raise polyclonal antibodies and lok, which one will best bind your epitope

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by JackBean
Tue Jan 18, 2011 11:06 am
 
Forum: General Discussion
Topic: simple epitope
Replies: 5
Views: 1970
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