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Enzyme stability

Enzyme stability

The extent to which an enzyme retains its structural conformation or its activity when subjected to storage, isolation, and purification or various other physical or chemical manipulations, including proteolytic enzymes and heat.

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DNase I stability

... So that after, say, 2 years it would be around 60 to 65 % or so? Though it seems that in low concentrations (or at low protein levels?) the enzyme is less stable. It also appears to be quite easily inactivated under mechanical stress, because it can happen even when vigorously pipetting ...

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by biohazard
Tue Nov 06, 2012 9:30 am
Forum: Molecular Biology
Topic: DNase I stability
Replies: 1
Views: 1988

Recombination in RNA viruses,Ruzic,Kovac

... This template-switching mechanism is fundamentally different from the enzyme-driven breakage±rejoining mechanism of homologous recombination in ... Desport, M., Collins, M. E. & Brownlie, J. (1998). Genome instability in BVDV: an examination of the sequence and structural in¯uences ...

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by anakovacevic
Sat Jun 12, 2010 8:28 pm
Forum: Cell Biology
Topic: Recombination in RNA viruses,Ruzic,Kovac
Replies: 2
Views: 44287

Re: Denaturation of enzymes

... conformation? Or could it be due to the free energy. Once a protein or enzyme is denatured, there is an excess of free energy, correct? What if, according to the stability of individual molecules and individual sections, that bonds reform in a different ...

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by carmenh
Fri Feb 27, 2009 10:21 pm
Forum: General Discussion
Topic: Denaturation of enzymes
Replies: 3
Views: 103793

Re: How to knock down a gene?

... RNA transcripts) form duplexes with RNA that are recognized by the enzyme RNase-H, which cleaves the RNA strand. RNase-independent oligos bind ... All of these antisense types differ in characteristics such as stability, specificity, efficacy and lack of non-antisense effects like innate ...

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by jonmoulton
Fri Oct 31, 2008 6:46 pm
Forum: Genetics
Topic: How to knock down a gene?
Replies: 1
Views: 4896

help, problem

... on the Activity of Proteolytic Enzymes Principle The ability of enzymes to accelerate biochemical reactions in mild conditions opens up many ... The very alkaline conditions [pH 10¬-11] reduced the activity and stability of the added enzymes and the product was not a success. By 1961, ...

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by martyn
Mon Apr 16, 2007 3:36 pm
Forum: Molecular Biology
Topic: help, problem
Replies: 3
Views: 4130
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