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Endotoxins

Endotoxins

toxins closely associated with the living cytoplasm or cell wall of certain microorganisms, which do not readily diffuse into the culture medium, but are released upon lysis of the cells.


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Re:

I think you cannot remove endotoxins solely by centrifugation, at least in any practical way. However, some substances allow you to precipitate them and the remove by centrifugation. Even this doesn't guarantee 100% endotoxin-free product ...

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by Nate3000
Wed Jun 11, 2008 6:46 pm
 
Forum: Microbiology
Topic: Bacteriophages and Acne?
Replies: 9
Views: 12619

Bacteriophages and Acne?

I think you cannot remove endotoxins solely by centrifugation, at least in any practical way. However, some substances allow you to precipitate them and the remove by centrifugation. Even this doesn't guarantee 100% endotoxin-free product ...

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by biohazard
Wed Jun 11, 2008 12:49 pm
 
Forum: Microbiology
Topic: Bacteriophages and Acne?
Replies: 9
Views: 12619

Bacteriophages and Acne?

... solution. This debris can prove fatal to humans, a problem that the purification technologies of the early days were unable to solve. Those are endotoxins, right? And can you get rid of them with a centrifuge? (Or do you even need to? I know the body can handle them when bacteriophages work ...

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by Nate3000
Tue May 27, 2008 5:46 am
 
Forum: Microbiology
Topic: Bacteriophages and Acne?
Replies: 9
Views: 12619

Contaminations in tissue culture

... even though finding a fly from your culture dish must be both very rare and extremely embarrassing! Finally, there can be chemical contaminants or endotoxins e.g. in the growth medium or some other reagents. Most well-known suppliers have very strict quality controls, but if you, say, buy your ...

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by biohazard
Thu Apr 24, 2008 8:35 am
 
Forum: Cell Biology
Topic: Contaminations in tissue culture
Replies: 7
Views: 3244

The Fiber Disease

... either in whole form, or by genetic alteration within the human organism? It seems to form crystals of proteinaceous insecticidal δ-endotoxins (Cry toxins) within its hosts. Some have suggested that our bodies are forming crystals. Any relation?

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by Frank N Stein
Thu Dec 28, 2006 2:27 pm
 
Forum: Human Biology
Topic: The Fiber Disease
Replies: 7403
Views: 5429364
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