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Endosome

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Propagation and Transfection of BHK cells?

... biotech company from Germany and have developed a new kind of transfection reagent called Viromer. Its a synthetic polymer , zero charged with an endosome escape mechanism. The advantages compared to other transfection reagents are: high transfection efficiency in hard to transfect cells like ...

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by OliviaZ
Tue Apr 08, 2014 10:48 am
 
Forum: Molecular Biology
Topic: Propagation and Transfection of BHK cells?
Replies: 2
Views: 1616

Overexpression

... biotech company from Germany and have developed a new kind of transfection reagent called Viromer. Its a synthetic polymer , zero charged with an endosome escape mechanism. The advantages compared to other transfection reagents are: high transfection efficiency in hard to transfect cells like ...

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by OliviaZ
Tue Apr 08, 2014 10:45 am
 
Forum: Molecular Biology
Topic: Overexpression
Replies: 2
Views: 1808

Re: Protein expression but no mRNA

... that the protein is being endocytosed rather than expressed in the cultured primary cells? I doubt that would be the case because on fusion of the endosome with a lysosome the proteins in the vesicle are usually degraded by the lysosomal enzymes. For the protein to enter using endosomes, it would ...

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by jonmoulton
Wed Feb 22, 2012 4:13 pm
 
Forum: Molecular Biology
Topic: Protein expression but no mRNA
Replies: 10
Views: 7103

Re:

I would guess the bacteria ends up in an endosome or lysosome. The energy is probably utilized in mitochondia. Ok, does anyone know what the other 2 organelles might be? Thanks, Petlover48

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by petlover48
Wed Oct 26, 2011 5:57 pm
 
Forum: Human Biology
Topic: What are the 4 organelles involved in this process ........?
Replies: 3
Views: 2631

What are the 4 organelles involved in this process ........?

I would guess the bacteria ends up in an endosome or lysosome. The energy is probably utilized in mitochondia.

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by JackBean
Wed Oct 26, 2011 5:45 pm
 
Forum: Human Biology
Topic: What are the 4 organelles involved in this process ........?
Replies: 3
Views: 2631
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